PXD038043

PXD038043 is an original dataset announced via ProteomeXchange.

Title	Direct comparison between a Burkholderia cenocepacia glycosylation mutant, and a CRISPRi-mediated glycosylation knockdown strain
Description	To determine whether CRISPR interference can be used to recapitulate a glycosylation null mutant strain in Burkholderia cenocepacia via data independent acquisition mass spectrometry using a low level of dCas9 induction. A guideless plasmid was containing in the wildtype and pglL mutant strains, and the strains harbouring the dCas9 insertion containing a pglL-targeting plasmid Bc-pglL-C, non-target, and the guideless plasmid.
HostingRepository	PRIDE
AnnounceDate	2023-03-21
AnnouncementXML	Submission_2023-03-20_18:27:51.068.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JessicaLewis
SpeciesList	scientific name: Burkholderia cenocepacia K56-2Valvano; NCBI TaxID: 985076;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-08 08:02:56	ID requested
⏵ 1	2023-03-20 18:27:51	announced

Dataset with its publication pending

submitter keyword: glycosylation, DIA, CRISPR interference, Burkholderia cenocepacia, CRISPRi,: Burkholderia cepacia complex

NichollasScott
contact affiliation	Department of Microbiology and Immunology, The University of Melbourne, Victoria, Australia
contact email	nichollas.scott@unimelb.edu.au
lab head
JessicaLewis
contact affiliation	University of Melbourne
contact email	jessica.lewis1@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038043

PRIDE project URI

• PRIDE
  1. PXD038043
     1. Label: PRIDE project
     2. Name: Direct comparison between a Burkholderia cenocepacia glycosylation mutant, and a CRISPRi-mediated glycosylation knockdown strain