PXD038009

PXD038009 is an original dataset announced via ProteomeXchange.

Title	Proteomic characteriaztion of the high light response of Calredoxin mutants (C. reinhardtii)
Description	Calredoxin (CRX) is a calcium (Ca2+)-dependent thioredoxin (TRX) in the chloroplast of Chlamydomonas reinhardtii. To further elucidate the function of CRX, we performed in-depth quantitative proteomics taking advantage of a crx insertional mutant (IMcrx), two CRISPR/Cas9 KO mutants, CRX rescues and wild type strains. These analyses revealed that the chloroplast NADPH-dependent TRX reductase (NTRC) is co-regulated with CRX. Electron transfer measurements revealed that CRX inhibits NADPH-dependent reduction of oxidized chloroplast 2-Cys peroxiredoxin (PRX1) via NTRC and that the function of the NADPH-NTRC complex is under strict control of CRX. Our data also demonstrated via non-reducing SDS-PAGE assays and redox proteomics that PRX1, Transketolase (TRK1), ATPC and Proton Gradient Related like 1 (PGRL1) are more oxidized under high light (HL) conditions in the absence of CRX. The redox tuning of PRX1 and control of the NADPH-NTRC complex via CRX interconnects redox control with active photosynthetic electron transport and metabolism as well as Ca2+ signaling. In this way, an economic use of NADPH for PRX1 reduction is assured. The finding, that in the absence of CRX, light-driven CO2 fixation is severely inhibited under HL conditions underpins the importance of CRX for redox tuning as well as for efficient photosynthesis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:47:27.733.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038009
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Martin Scholz
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-07 09:55:33	ID requested
1	2023-10-04 10:47:29	announced
⏵ 2	2023-11-14 07:47:28	announced	2023-11-14: Updated project metadata.

10.6019/PXD038009;

submitter keyword: Chlamydomonas, algae, high light, photosynthesis, peroxiredoxin,Calredoxin

Michael Hippler
contact affiliation	IBBP, WWU Muenster
contact email	mhippler@uni-muenster.de
lab head
Martin Scholz
contact affiliation	IBBP Muenster
contact email	mscholz@uni-muenster.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038009
     1. Label: PRIDE project
     2. Name: Proteomic characteriaztion of the high light response of Calredoxin mutants (C. reinhardtii)