PXD037991

PXD037991 is an original dataset announced via ProteomeXchange.

Title	Opisthorchis felineus excretory-secretory product (ESP) and lysate proteomics by GeLC-MS / MS
Description	A significant number of pathological conditions, accompanied by chronic non-healing wounds, demands searching for new modern therapeutic approaches. Well-documented ability of O. felineus to initiate extracellular matrix (ECM) remodeling and liver epithelium regeneration suggests that its bioactive molecules may stimulate skin wound healing processes. The aim of this study was to investigate the wound healing potential of the Opisthorchis felineus excretory-secretory and lysate proteins on a murine model. The following methods were used for the study: histological (wounded skin condition), immunohistochemical (ECM, neoangiogenesis, O. felineus GST and TPx proteins), gene expression analysis (inflammation, angiogenesis, ECM condition). O. felineus excretory-secretory product (ESP) and lysate proteins have revealed wound-healing potential, they: i) reduce inflammation levels, ii) modulate vascular response, iii) stimulate collagen deposition and dermal ECM remodeling. Additional proteomic analysis of adult O. felineus ESP and lysate samples was conducted. Proteomic analysis approach called GeLC-MS / MS was chosen to study of the excretory-secretory product and lysate proteins. This approach is based on one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), in-gel protein digestion with trypsin followed by liquid chromatography-tandem mass spectrometry. The SDS-PAGE step allow to removes hemozoin also as detergents, buffers and salts from the protein extract that may interfere with mass spectrometry analysis
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:55.344.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olga Tikhonova
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-07 09:00:38	ID requested
1	2023-03-11 11:22:59	announced
⏵ 2	2023-11-14 08:43:57	announced	2023-11-14: Updated project metadata.

Kovner AV, Tarasenko AA, Zaparina O, Tikhonova OV, Pakharukova MY, Mordvinov VA, Wound healing approach based on excretory-secretory product and lysate of liver flukes. Sci Rep, 12(1):21639(2022) [pubmed]

submitter keyword: Opisthorchis felineus, SDS-PAGE, excretory-secretory product, lysate, LC-MS/MS

Olga Tikhonoiva
contact affiliation	Institute of Biomedical Chemistry, "Human Proteome" Core Facility Centre
contact email	ovt.facikity@gmail.com
lab head
Olga Tikhonova
contact affiliation	Institute of Biomedical Chemistry
contact email	ovt.facility@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037991
     1. Label: PRIDE project
     2. Name: Opisthorchis felineus excretory-secretory product (ESP) and lysate proteomics by GeLC-MS / MS