PXD037983

PXD037983 is an original dataset announced via ProteomeXchange.

Title	Regioselective Protein Oxidative Cleavage Enabled by an Enzyme-like Recognition of an Inorganic Metal Oxo Cluster Ligand
Description	Metal-catalyzed oxidation reactions (MCO) is one of the most applicable methods to induce protein oxidative modifications (e.g., protein oxidation, protein oxidative cleavage and crosslinking), which are essential techniques for many applications in biotechnology and redox proteomics. However, a suitable ligand is needed to assure high stability and enhance the metal catalytic efficiency in order to induce these oxidative modifications in a selective manner. Herein, we report the influence of the polyoxometalates (POMs) as inorganic ligands on the catalytic efficiency of Cu ions to induce oxidative cleavage of a protein. In the presence of ascorbic acid as (Asc), the Cu-substituted POM (Cu-POM), α2-K8P2W17O61(Cu2+.OH2), (CuWD), cleaved hen egg white lysozyme (HEWL), a protein consisting of 129 amino acids, producing only four peptide fragments. Analyzing the intact protein, in absence and presence of CuWD/Asc, and the peptide four peptide fragments via nLC-MS/MS revealed that CuWD/Asc induced oxidative modifications and cleavage at and/or near CuWD/HEWL binding sites
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:51.426.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037983
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tamara Vasovic
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-07 08:27:37	ID requested
1	2023-01-30 10:08:43	announced
⏵ 2	2023-11-14 08:59:51	announced	2023-11-14: Updated project metadata.

10.6019/PXD037983;

submitter keyword: Hen egg white lysosime, LC-MS/MS

Tanja Ćirković Veličković
contact affiliation	PhD Professor of Biochemistry & Corresponding member ofSerbian Academy of Sciences and Arts University of Belgrade-Faculty of Chemistry, Center of Excellence for Molecular Food Sciences and Department of Biochemistry, Studentski trg 12-16, 11 000 Belgrade, Serbia. Professor of Food Chemistry Department of Environmental Technology, Food Technology and Molecular Biotechnology, Ghent University Global Campus, Yeonsu-Gu, 21985 Incheon, Republic of Korea
contact email	tcirkov@chem.bg.ac.rs
lab head
Tamara Vasovic
contact affiliation	University of Belgade - Faculty of Chemistry
contact email	tamara.vasovic@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD037983

PRIDE project URI

• PRIDE
  1. PXD037983
     1. Label: PRIDE project
     2. Name: Regioselective Protein Oxidative Cleavage Enabled by an Enzyme-like Recognition of an Inorganic Metal Oxo Cluster Ligand