PXD037977

PXD037977 is an original dataset announced via ProteomeXchange.

Title	Dataset from The proteome landscape of the black soldier fly provides insights into protein function and microbial diversity
Description	The larvae of black soldier fly (BSF) Hermetia illucens (Diptera: Stratiomyidae), has demonstrated ability in the efficient bioconversion of organic waste into a sustainable source of food and feed, but fundamental biology remains to be discovered to exploit their full biodegradative potential. Herein, LC-MS/MS was used to assess the efficiency of eight differing extraction protocols to build foundational knowledge regarding the proteome landscape of both BSF larvae body and gut. No specific protocol was superior in capturing the BSF body and gut proteome, but each yielded complementary information to improve BSF proteome coverage. Protocol-specific functional annotation using protein level information has shown that the selection of extraction buffer can affect protein detection and their associated functional classes within the measured BSF larval gut proteome. Metaproteome analysis on BSF larvae gut has uncovered the prevalence of two bacterial phyla: actinobacteria and proteobacteria. We envisage that comparing a range of extraction protocols and investigating the proteome from the BSF body and gut separately will expand the fundamental knowledge of the BSF proteome and thereby provide translational opportunities for future research to enhance their efficiency for waste degradation and contribution to the circular economy.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:40:04.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophia Escobar Correas
SpeciesList	scientific name: Hermetia illucens; NCBI TaxID: 343691;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-07 08:23:45	ID requested
1	2023-03-11 01:39:06	announced
⏵ 2	2023-11-14 08:40:08	announced	2023-11-14: Updated project metadata.

Bose U, Juhasz A, Stockwell S, Escobar-Correas S, Marcora A, Paull C, Broadbent JA, Wijffels G, Unpacking the Proteome and Metaproteome of the Black Soldier Fly Larvae: Efficacy and Complementarity of Multiple Protein Extraction Protocols. ACS Omega, 8(8):7319-7330(2023) [pubmed]

submitter keyword: proteomics,Black soldier fly, microbiota, proteome

Utpal Bose
contact affiliation	CSIRO Agriculture and Food, 306 Carmody Rd, St Lucia QLD 4067, Australia
contact email	utpal.bose@csiro.au
lab head
Sophia Escobar Correas
contact affiliation	CSIRO
contact email	sophia.escobarcorreas@csiro.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037977

PRIDE project URI

• PRIDE
  1. PXD037977
     1. Label: PRIDE project
     2. Name: Dataset from The proteome landscape of the black soldier fly provides insights into protein function and microbial diversity