PXD037959

PXD037959 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of proteins related to defense responses in Arabidopsis plants transformed with the rolB oncogene
Description	During Agrobacterium rhizogenes–plant interaction, the rolB gene is transferred into the plant genome and is stably inherited in the plant’s offspring. Among the numerous effects of rolB on plant metabolism, including the activation of secondary metabolism, its effect on plant defense systems has not been sufficiently studied. Here, we performed a proteomic analysis of rolB-expressing Arabidopsis plants with a particular focus on defense proteins. In rolB plants, a reduced amount of scaffold proteins RACK1A, RACK1B, and RACK1C, known as receptors for activated C-kinase 1, was found. Proteomic analysis showed that rolB could potentially suppress the plant immune system by suppressing the RNA-binding proteins GRP7, CP29B, and CP31B, similar to the action of type III bacterial effectors. At the same time, rolB plants induce massive biosynthesis of protective proteins VSP1, VSP2, and PR4, which are markers of the activated jasmonate pathway. Moreover, rolB plants activate all components of the PYK10 defense complex of the endoplasmic reticulum involved in the metabolism of glucosinolates. We hypothesized that various defense systems activated by rolB are aimed at protecting the host plant from competing phytopathogens in order to create an effective ecological niche for A. rhizogenes. A RolB → RACK1A signaling module has been proposed that can exert most of the rolB-mediated effects on plant physiology and biochemistry.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:15.583.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037959
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dmitriy Bulgakov
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-04 09:19:46	ID requested
1	2023-03-11 10:18:58	announced
⏵ 2	2023-11-14 08:43:16	announced	2023-11-14: Updated project metadata.

Vereshchagina YV, Mironova AA, Bulgakov DV, Bulgakov VP, Oncogene. Int J Mol Sci, 24(3):(2023) [pubmed]

10.6019/PXD037959;

submitter keyword: Arabidopsis, proteomics,RolB plant oncogene, plant defense, RACK1A, PYK10 complex

Victor P. Bulgakov
contact affiliation	Lab. of Bioengineering, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 159 Stoletiya Street, Vladivostok, 690022, Russia
contact email	bulgakov@biosoil.ru
lab head
Dmitriy Bulgakov
contact affiliation	Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok-22, 690022, Russia
contact email	bulgakov-dv@mail.ru
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037959
     1. Label: PRIDE project
     2. Name: Proteomic analysis of proteins related to defense responses in Arabidopsis plants transformed with the rolB oncogene