PXD037954

PXD037954 is an original dataset announced via ProteomeXchange.

Title	Micro-topographies induce epigenetic reprogramming and quiescence in human mesenchymal stem cells
Description	Biomaterials can control cell and nuclear morphology. Since the shape of the nucleus influences chromatin architecture, gene expression, and cell identity, surface topography can control cell phenotype. This study explores how surface topography influences nuclear morphology, histone modifications, and expression of histone-associated proteins through advanced histone mass spectrometry and microarray analysis. We found that nuclear confinement is associated with loss of both histone acetylation and nucleoli abundance, while pathway analysis revealed a substantial reduction in gene expression associated with chromosome organization. In light of previous observations where we found a decrease in proliferation and metabolism induced by micro-topographies, we connect these findings with a quiescent phenotype in mesenchymal stem cells, as further shown by a reduction of ribosomal proteins and the maintenance of multipotency on micro-topographies after long-term culture conditions. Furthermore, this influence of micro-topographies on nuclear morphology and proliferation was reversible, as shown by a full return of proliferation when re-cultured on a flat surface. Our findings provide novel insights on how biophysical signaling influences nuclear organization and subsequent cellular phenotype.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:14:31.993.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maarten Dhaenens
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; acetylated residue; formylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-04 09:05:17	ID requested
1	2022-12-03 11:36:04	announced
⏵ 2	2023-11-14 08:14:35	announced	2023-11-14: Updated project metadata.

Vermeulen S, Van Puyvelde B, Bengtsson Del Barrio L, Almey R, van der Veer BK, Deforce D, Dhaenens M, de Boer J, Micro-Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells. Adv Sci (Weinh), 10(1):e2203880(2022) [pubmed]

submitter keyword: Nucleus, epigenetics, biomaterials, mesenchymal stem cells, mechanobiology, DDA

Maarten Dhaenens
contact affiliation	ProGenTomics, Laboratory of Pharmaceutical Biotechnology,Ghent University
contact email	maarten.dhaenens@ugent.be
lab head
Maarten Dhaenens
contact affiliation	Faculity of Pharmaceutical Biotechnology
contact email	maarten.dhaenens@ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD037954

PRIDE project URI

• PRIDE
  1. PXD037954
     1. Label: PRIDE project
     2. Name: Micro-topographies induce epigenetic reprogramming and quiescence in human mesenchymal stem cells