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PXD037950

PXD037950 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSpatially Resolved Proteomics via Tissue Expansion
DescriptionSpatially resolved proteomics is an emerging approach, however, it remains technically challenging due to the complexity of the tissue microsampling techniques and mass spectrometry analysis of nanoscale specimen volumes. Here, we describe an expansion proteomics method ProteomEx, a combination of tissue expansion with mass spectrometry-based proteomics, which enables quantitative assessment of the spatial variability of the proteome in mammalian tissues at ~125 µm lateral resolution equivalent to the tissue volume of 0.7 nL using manual microsampling without the need for a custom or special equipment. We validated and demonstrated the utility of ProteomEx for high-throughput large-scale proteomics profiling of biological tissues including brain, liver, and breast cancer. We further applied ProteomEx for identifying proteins associated with Alzheimer's disease in a mouse model by comparative proteomic analysis of brain subregions.
HostingRepositoryiProX
AnnounceDate2022-11-04
AnnouncementXMLSubmission_2023-01-31_19:59:52.010.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLu Li
SpeciesList scientific name: Mus ; NCBI TaxID: 10088;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-03 21:19:34ID requested
12022-11-03 21:20:45announced
22023-01-31 19:59:57announced2023-02-01: Update publication information.
Publication List
Li L, Sun C, Sun Y, Dong Z, Wu R, Sun X, Zhang H, Jiang W, Zhou Y, Cen X, Cai S, Xia H, Zhu Y, Guo T, Piatkevich KD, Spatially resolved proteomics via tissue expansion. Nat Commun, 13(1):7242(2022) [pubmed]
Keyword List
submitter keyword: tissue expansion, spatial proteomics, data-independent acquisition, mass spectrometry, Alzheimer's disease
Contact List
Tiannan Guo
contact affiliationWestlake University
contact emailguotiannan@westlake.edu.cn
lab head
Lu Li
contact affiliationWestlake University
contact emaillilulilu777@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI