PXD037909

PXD037909 is an original dataset announced via ProteomeXchange.

Title	Energy metabolism in skeletal muscle cells from donors with different body mass index
Description	Obesity and physical inactivity have a profound impact on skeletal muscle metabolism. In the present work, we have investigated differences in protein expression and energy metabolism in primary human skeletal muscle cells established from lean donors (BMI<25 kg/m2) and individuals with obesity (BMI>30 kg/m2). Furthermore, we have studied the effect of fatty acid pretreatment on energy metabolism in myotubes from these donor groups. Alterations in protein expression were investigated using proteomic analysis, and energy metabolism was studied using radiolabeled substrates. Gene Ontology enrichment analysis showed that glycolytic, apoptotic, and hypoxia pathways were upregulated, whereas the pentose phosphate pathway was downregulated in myotubes from donors with obesity compared to myotubes from lean donors. Moreover, fatty acid, glucose, and amino acid uptake were increased in myotubes from individuals with obesity. However, fatty acid oxidation was reduced, glucose oxidation was increased in myotubes from subjects with obesity compared to cells from lean. Pretreatment of myotubes with palmitic acid (PA) or eicosapentaenoic acid (EPA) for 24 h increased glucose oxidation and oleic acid uptake. EPA pretreatment increased the glucose and fatty acid uptake and reduced leucine fractional oxidation in myotubes from donors with obesity. In conclusion, these results suggest that myotubes from individuals with obesity showed increased fatty acid, glucose, and amino acid uptake compared to cells from lean donors. Furthermore, myotubes from individuals with obesity had reduced fatty acid oxidative capacity, increased glucose oxidation, and a higher glycolytic reserve capacity compared to cells from lean donors. Fatty acid pretreatment enhances glucose metabolism, and EPA reduces oleic acid and leucine fractional oxidation in myotubes from donor with obesity, suggesting increased metabolic flexibility after EPA treatment
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:54.752.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sachin Singh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF fleX

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-03 04:22:21	ID requested
1	2023-03-11 05:18:46	announced
⏵ 2	2023-11-14 08:43:01	announced	2023-11-14: Updated project metadata.

Katare PB, Dalmao-Fernandez A, Mengeste AM, Hamarsland H, Ellefsen S, Bakke HG, Kase ET, Thoresen GH, Rustan AC, Energy metabolism in skeletal muscle cells from donors with different body mass index. Front Physiol, 13():982842(2022) [pubmed]

submitter keyword: metabolism, obesity,skeletal muscle cells, mitochondria, eicosapentaenoic acid

Parmeshwar B. Katare
contact affiliation	Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, Norway
contact email	p.b.katare@farmasi.uio.no
lab head
Sachin Singh
contact affiliation	Proteomics core facility, Olso University hospital, Norway
contact email	sachin.kumar.singh@rr-research.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037909
     1. Label: PRIDE project
     2. Name: Energy metabolism in skeletal muscle cells from donors with different body mass index