PXD037907

PXD037907 is an original dataset announced via ProteomeXchange.

Title	TMTC1 promotes invasiveness of ovarian cancer
Description	Ovarian cancer is the most lethal gynecological malignancy and is characterized by peritoneal disseminated metastasis. Although O-mannosyltransferase TMTC1 is highly expressed by ovarian cancer, its pathophysiologic role in ovarian cancer remains unclear. Here, immunohistochemistry showed that TMTC1 was overexpressed in ovarian cancer tissues compared with the adjacent non-tumor ovarian tissues and high TMTC1 expression was associated with poor prognosis in patients with ovarian cancer. Silencing TMTC1 reduced ovarian cancer cell viability, migration, and invasion in vitro as well as suppressed peritoneal tumor growth and metastasis in vivo. Moreover, TMTC1 knockdown reduced cell-laminin adhesion, which was associated with the decreased phosphorylation of FAK at pY397. Conversely, TMTC1 overexpression promoted these malignant properties in ovarian cancer cells. Glycoproteomic analysis and Concanavalin A (ConA) pull-down assays showed that integrins β1 and β4 were novel O-mannosylated protein substrates of TMTC1. Furthermore, TMTC1-mediated cell migration and invasion were significantly reversed by siRNA-mediated knockdown of integrin β1 or β4. Collectively, these results suggest that TMTC1-mediated invasive behaviors are primarily through integrins β1 and β4 and that TMTC1 is a potential therapeutic target for ovarian cancer.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:28.878.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hsin-Yi Wu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	mannosylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-03 04:19:25	ID requested
1	2023-10-24 11:19:16	announced
⏵ 2	2023-11-14 09:07:29	announced	2023-11-14: Updated project metadata.

Yeh TC, Lin NY, Chiu CY, Hsu TW, Wu HY, Lin HY, Chen CH, Huang MC, 4. Cancer Gene Ther, 30(8):1134-1143(2023) [pubmed]

submitter keyword: O-mannosylation, Ovarian cancer,TMTC1

Min-Chuan Huang
contact affiliation	Graduate Institute of Anatomy and Cell Biology, College of Medicine, National Taiwan University
contact email	mchuang@ntu.edu.tw
lab head
Hsin-Yi Wu
contact affiliation	National Taiwan University
contact email	clairewu.wu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD037907

PRIDE project URI

• PRIDE
  1. PXD037907
     1. Label: PRIDE project
     2. Name: TMTC1 promotes invasiveness of ovarian cancer