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PXD037904

PXD037904 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhoneutria nigriventer venom profiling using high-throughput ion channel screen and structural venomics reveals highly complex composition and novel neuroactive peptides
DescriptionSpider venoms are a unique source of bioactive peptides displaying remarkable structural stability and potent neuroactivity. Phoneutria nigriventer, often referred to as Brazilian wandering spider, banana spider or “armed” spider, is endemic from South America and amongst the most dangerous venomous spiders in the world. Envenomation accidents with P. nigriventer often occur in Brazil with approximately 4,000 cases per year and which symptoms include priapism, hypertension, blurred vision, sweating, and vomiting, amongst others. Besides its clinical relevance, P. nigriventer venom comprises promising peptide drug leads providing therapeutic effects in a range of disease models. In this study, we further explored the neuroactivity and molecular diversity of the venom from P. nigriventer using fractionation-guided high-throughput cellular assays coupled to proteomics analysis and multi-pharmacology activity to broaden the knowledge and therapeutic potential of this venom, as well as a proof-of-concept for an investigative pipeline to study spider-venom derived neuroactive peptides. We applied ion channel assays in a neuroblastoma cell line to investigate modulation of voltage-gated sodium and calcium channels, and nicotinic acetylcholine α7 receptor. We then investigated the venom components using mass spectrometry to identify peptide masses and sequences associated to the observed neuromodulations. Our findings showed this venom is highly complex compared to other neurotoxin-rich venoms and comprises potent modulators of voltage-gated sodium and calcium channels which were classified into 4 families of neuroactive peptides based on their activities and structures. In addition to the reported P. nigriventer neuroactive peptides, we identified at least 27 novel cysteine-rich venom peptides in which neuroactivities are still to be elucidated in voltage-gated ion channels and other potential targets. Our findings provide a new basis for studying non-explored bioactivities of known and novel neuroactive components in P. nigriventer venom, and further supports the successful application of our discovery pipeline for identifying ion channel-targeting venom peptides with potential to become drug leads with diverse exploratory and therapeutic applications.
HostingRepositoryPRIDE
AnnounceDate2023-02-09
AnnouncementXMLSubmission_2023-02-08_16:23:04.764.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndrewWalker
SpeciesList scientific name: Phoneutria nigriventer; NCBI TaxID: 6918;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-03 04:18:32ID requested
12023-02-08 16:23:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: venom peptide, wandering spider,spider venom
Contact List
Glenn F.King
contact affiliationInstitute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4122, Australia
contact emailglenn.king@imb.uq.edu.au
lab head
AndrewWalker
contact affiliationUniversity of Queensland
contact emaila.walker@uq.edu.au
dataset submitter
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Dataset FTP location
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