PXD037886

PXD037886 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cilia-APEX proteomics profiling of Arl6-/- vs wiltype reveal UbK63 retrieval adaptors
Description	Ascrobate peroxidase (APEX) was expressed as a fusion protein and directed to primary cilia of mouse inner medullary collecting duct (mIMCD-3) cells, where it was used for proximity labeling to biotinylate proteins, which are subsequently enriched by streptavidin chromatography and identified by mass spectrometry. Cilia-APEX proteomics profiling compared the cilia proteomes of wild-type and Arl6-/- cilia (generated by CRISPR/Cas9-mediated genome editing) by spectral counting.
HostingRepository	MassIVE
AnnounceDate	2023-03-02
AnnouncementXML	Submission_2023-03-02_16:14:25.766.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Mick
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-02 09:39:28	ID requested
⏵ 1	2023-03-02 16:14:26	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Proximity Labeling Proteomics, cilia-APEX profiling

submitter keyword: Proximity Labeling Proteomics, cilia-APEX profiling

Contact List

Maxence V. Nachury
contact affiliation	University of California San Francisco
contact email	maxence.nachury@ucsf.edu
lab head
David Mick
contact affiliation	Saarland University School of Medicine
contact email	david.mick@uks.eu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090633/