PXD037838

PXD037838 is an original dataset announced via ProteomeXchange.

Title	CUX1 recruits SWI/SNF to promote chromatin accessibility in human hematopoietic cells
Description	Transcription factors(TF) plays a key role in hematopoiesis via promoting cell-type specific gene expression. A subset of TFs, canonically termed “pioneer factors”, possesses the ability to bind to otherwise inaccessible heterochromatin regions, and recruit additional TFs, co-activators, and chromatin remodelers to establish activating/repressive cis-regulatory elements(CRE). To date, few of these TFs with pioneer activity has been elucidated in hematopoiesis. CUX1, a homeobox containing TF frequently mutated in a range of hematological malignancies, has been reported to regulate essential cellular processes and regulate myeloid vs erythroid differentiation. However, the molecular mechanism governing its transcriptional function remains unclear. Using a multi-faceted functional genomic approach, we discovered that CUX1 interacts and recruits the chromatin remodeler SWI/SNF to distal enhancers in both lymphoblast K562 cell line and primary human HSPC. The interaction promotes an accessible state of chromatin, changes in nucleosome positioning and transcriptional programs. We also discovered that the CUX1 & SWI/SNF binding sites are co-occupied by other hematopoietic transcriptional factors. Together this revealed a unique mechanism of CUX1’s regulatory function in hematopoietic development: it recruits SWI/SNF chromatin remodeler to distal enhancers, establish an accessible chromatin landscape and enabled other hematopoietic TFs to bind and activates cell-type specific gene expression programs.
HostingRepository	PRIDE
AnnounceDate	2026-03-16
AnnouncementXML	Submission_2026-03-16_03:54:07.808.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-31 07:56:48	ID requested
⏵ 1	2026-03-16 03:54:09	announced

10.1016/j.celrep.2024.114227;

Liu W, Kurkewich JL, Stoddart A, Khan S, Anandan D, Gaubil AN, Wolfgeher DJ, Jueng L, Kron SJ, McNerney ME, CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex. Cell Rep, 43(5):114227(2024) [pubmed]

submitter keyword: Human, Q-Exactive, IP-MS, Cux1, SWI/SNF, Chromatin

Dr. Megan E. McNerney M.D.Ph.D.
contact affiliation	Committee on Cancer Biology, The University of Chicago, Chicago, IL, USA Department of Pathology, The University of Chicago, Chicago, IL, USA Department of Pediatrics, The University of Chicago, Chicago, IL, USA The University of Chicago Medicine Comprehensive Cancer Center, University of Chicago, Chicago, IL, USA
contact email	Megan.McNerney@uchospitals.edu
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD037838

PRIDE project URI

• PRIDE
  1. PXD037838
     1. Label: PRIDE project
     2. Name: CUX1 recruits SWI/SNF to promote chromatin accessibility in human hematopoietic cells