PXD037837

PXD037837 is an original dataset announced via ProteomeXchange.

Title	Immunopeptidomics reveals determinants of Mycobacterium tuberculosis antigen presentation on MHC class I (DDA data)
Description	Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is a leading cause of infectious disease mortality worldwide for which no sufficiently protective vaccine exists. CD8+ T cells contribute to protective immunity to TB, but the antigenic targets presented on MHC-I by macrophages infected with virulent Mtb for recognition by CD8+ T cells have not been conclusively defined. Here, we use mass spectrometry to directly identify Mtb-derived peptides presented on MHC class I (MHC-I) by infected primary human monocyte-derived macrophages. We identified 16 MHC-I peptides derived from 12 Mtb proteins, and validated these identifications using internal standard parallel reaction monitoring (SureQuant). Our results show that this set of antigens is highly enriched for substrates of type VII secretion systems (T7SS) relative to the Mtb proteome. Our results identify specific Mtb proteins that may be suitable targets for subunit vaccines designed to elicit protective CD8+ T cell-mediated immunity and suggest that T7SS substrates may be a fruitful class of antigens to prioritize for further vaccine target discovery efforts. Note: donors labeled A, C, D, E, H, and I in file names here are renamed sequentially (A, B, C, D, E, and F) in the manuscript describing this study to avoid confusion.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:52:27.940.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037837
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Owen Leddy
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-31 07:56:28	ID requested
1	2023-04-24 06:43:05	announced
⏵ 2	2023-11-14 06:52:28	announced	2023-11-14: Updated project metadata.

10.6019/PXD037837;

Leddy OK, White FM, Bryson BD, Leveraging Immunopeptidomics To Study and Combat Infectious Disease. mSystems, 6(4):e0031021(2021) [pubmed]

submitter keyword: macrophage, human,tuberculosis, vaccines, infectious disease, immunopeptidomics, phagocyte, immunopeptidome, MHC-I, tb

Forest White
contact affiliation	Massachusetts Institute of Technology
contact email	fwhite@mit.edu
lab head
Owen Leddy
contact affiliation	Massachusetts Institute of Technology
contact email	owenl@mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD037837

PRIDE project URI

• PRIDE
  1. PXD037837
     1. Label: PRIDE project
     2. Name: Immunopeptidomics reveals determinants of Mycobacterium tuberculosis antigen presentation on MHC class I (DDA data)