PXD037834

PXD037834 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteome Analysis reveal the involvement of STEAP1 on vesicles-mediated transport in human LNCaP prostate cancer cells
Description	Prostate cancer (PCa) continues to be one of the most common cancers in men worldwide. The six transmembrane epithelial antigen of the prostate 1 (STEAP1) protein is overexpressed in several types of human tumours, particularly in PCa. Our research group has demonstrated that STEAP1 overexpression is associated with PCa progression and aggressiveness. Therefore, understanding the cellular and molecular mechanisms triggered by STEAP1 overexpression will provide important insights to delineate new strategies for PCa treatment. In the present work, a proteomic strategy was used to characterize the intracellular signalling pathways and the molecular targets downstream of STEAP1 in PCa cells. A label-free approach was applied using an Orbitrap LC-MS/MS system to characterize the proteome of STEAP1-knockdown PCa cells. More than 6700 proteins were identified, of which a total of 526 proteins were found differentially expressed in scramble siRNA versus STEAP1 siRNA (234 proteins up-regulated and 292 proteins down-regulated). Bioinformatics analysis allowed us to explore the mechanism through which STEAP1 exerts influence on PCa, revelling that endocytosis, RNA transport, apoptosis, aminoacyl-tRNA biosynthesis and metabolic pathways are the biological processes where STEAP1 is involved. By immunoblotting, it was confirmed that STEAP1 knockdown induced the up-regulation of cathepsin B, intersectin-1 and syntaxin 4,while it down-regulation HRas, PIK3C2A and DIS3. These finding suggested that blocking STEAP1 might be a strategy to activate apoptosis and endocytosis, and diminish cellular metabolism and intercellular communication, leading to inhibition of PCa progression.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:06.291.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037834
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fátima Santos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-31 07:55:18	ID requested
1	2023-10-18 09:43:05	announced
⏵ 2	2023-11-14 09:06:16	announced	2023-11-14: Updated project metadata.

Rocha SM, Santos FM, Socorro S, Passarinha LA, Maia CJ, Proteomic analysis of STEAP1 knockdown in human LNCaP prostate cancer cells. Biochim Biophys Acta Mol Cell Res, 1870(7):119522(2023) [pubmed]

10.6019/PXD037834;

submitter keyword: proteomics, prostate cancer, label-free quantification,STEAP1, endocytic pathway

Cláudio Jorge Maia
contact affiliation	CICS-UBI–Health Sciences Research Center, Universidade da Beira Interior, 6201-506 Covilhã, Portugal
contact email	cmaia@fcsaude.ubi.pt
lab head
Fátima Santos
contact affiliation	CNB
contact email	ftxsantos@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037834
     1. Label: PRIDE project
     2. Name: Quantitative Proteome Analysis reveal the involvement of STEAP1 on vesicles-mediated transport in human LNCaP prostate cancer cells