PXD037833

PXD037833 is an original dataset announced via ProteomeXchange.

Title	Exposure of Aspergillus fumigatus to Klebsiella pneumoniae culture filtrate inhibits growth and stimulates gliotoxin production.
Description	Aspergillus fumigatus is an opportunistic fungal pathogen of the lungs resulting in both chronic and acute infection. The fungus interacts with numerous bacteria that compose the microflora of the lung including Pseudomonas aeruginosa and Klebsiella pneumoniae, both common isolates from Cystic fibrosis sputum. It is well documented that bacterial and fungal interactions both physically and through production of secreted products which can alter the growth and virulence of other species. The interaction with P. aeruginosa has been characterised previously indicating decreased growth and increased gliotoxin production in the presence of cells and the opposite when only exposed to culture filtrate. In our analysis exposure of cell free culture filtrate inhibited fungal growth and increased gliotoxin production. This was supported with proteomic analysis indicating reduction in 1,3-beta-glucanosyltransferase (-3.97 fold), methyl sterol monooxygenase erg25B (-2.9 fold) and Calcium/calmodulin dependent protein kinase (-4.2 fold) involved in fungal development. Coupled with increased expression of Glutathione S-transferase gliG (6.17 fold), non-ribosomal peptide synthase gliP (3.67 fold), O-methyltransferase gliM (3.5 fold), Gamma-glutamyl acyltransferase gliK (2.89 fold) and thioredoxin reductase gliT (2.33 fold) involved in Gliotoxin production indicate a possible shift towards secondary metabolism. Qualitative proteomic analysis of the bacterial supernatant also elucidates possible mechanisms for these alterations including physical interaction by Outer membrane protein A, High-affinity zinc uptake system protein ZnuA and Iron uptake system component EfeO reducing essential nutrient availability, enzymatic activity including Periplasmic serine endoprotease DegP-like and redox active proteins including Alkyl hydroperoxide reductase C. These proteomic alterations to the fungus could have implications for the severity of virulence in the context of coinfection and may impact disease progression.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:28.500.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aaron Curtis
SpeciesList	scientific name: Aspergillus fumigatus Af293; NCBI TaxID: 330879;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-31 07:54:56	ID requested
1	2023-03-11 10:19:56	announced
⏵ 2	2023-11-14 08:43:37	announced	2023-11-14: Updated project metadata.

Curtis A, Ryan M, Kavanagh K, Culture Filtrate Inhibits Growth and Stimulates Gliotoxin Production. J Fungi (Basel), 9(2):(2023) [pubmed]

submitter keyword: Aspergillus, proteomics, gliotoxin, klebsiella, polymicrobial interaction

Professor Kevin Kavanagh
contact affiliation	Medical Mycology Laboratory, Department of Biology, Maynooth University
contact email	kevin.kavanagh@mu.ie
lab head
Aaron Curtis
contact affiliation	Maynooth Univeristy
contact email	aaron.curtis.2018@mumail.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037833
     1. Label: PRIDE project
     2. Name: Exposure of Aspergillus fumigatus to Klebsiella pneumoniae culture filtrate inhibits growth and stimulates gliotoxin production.