PXD037822

PXD037822 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic analysis of small extracellular vesicles purified by three methods
Description	To identify cellular senescence markers using senescent fibroblast-derived extracellular vesicles (EVs), we collected small EVs by three different methods: size exclusion chromatography (SEC), affinity column for phosphatidylserine (PS), and immunoprecipitation (IP) using antibodies against tetraspanin proteins (CD9, CD63 and CD81), from young and senescent fibroblasts to proteomic analysis. In addition, small EVs were collected from culture supernatants of cells derived from patients with Werner's syndrome, a known Progeria, and healthy individuals, and subjected to proteomic analysis.
HostingRepository	jPOST
AnnounceDate	2023-10-29
AnnouncementXML	Submission_2023-10-28_08:00:06.360.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tomoka Misawa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-28 19:48:22	ID requested
⏵ 1	2023-10-28 08:00:06	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: extracellular vesicles

submitter keyword: extracellular vesicles

Contact List

Akiko Takahashi
lab head
Tomoka Misawa
contact affiliation	Division of Cellular Senescence, The Cancer Institute, Japanese Foundation for Cancer Research
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001902/