PXD037813 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mouse osteoclast secretory lysosomes nLC-ESI-MS/MS - WT vs Slc37a2 KO |
Description | To survey the proteome of osteoclast secretory lysosomes, we used superparamagnetic iron oxide nanoparticles (SPIONs) to enrich for these endo-lysosomal-related organelles from murine osteoclast cultures. Briefly, large scale murine bone marrow monocyte (BMM)-derived osteoclast cultures were ‘pulsed’ with SPIONs to encourage uptake into endosomes and then ‘chased’ into secretory lysosomes upon the convergence of SPION-loaded endosomes with lysosomes and secretory pathways. Following the ‘pulse-chase’, osteoclasts were homogenized, SPION-loaded organelles captured-from post-nuclear supernatants using magnetic columns, and enriched organelles eluted and processed for 1D in-gel digestion and mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2023-01-25 |
AnnouncementXML | Submission_2023-01-24_18:39:56.511.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AmyRibet |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-28 07:38:11 | ID requested | |
⏵ 1 | 2023-01-24 18:39:56 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Lysosome, LRO, Mouse, SPION,Osteoclast, Secretory lysosome |
Contact List
NathanPavlos |
contact affiliation | The Bone Biology and Disease Laboratory, School of Biomedical Sciences, The University of Western Australia |
contact email | nathan.pavlos@uwa.edu.au |
lab head | |
AmyRibet |
contact affiliation | The University of Western Australia |
contact email | 21703693@student.uwa.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037813
- Label: PRIDE project
- Name: Mouse osteoclast secretory lysosomes nLC-ESI-MS/MS - WT vs Slc37a2 KO