PXD037811

PXD037811 is an original dataset announced via ProteomeXchange.

Title	Investigation of inter- and intra-day variability of tear fluid regarding flow rate, protein concentration as well as protein composition
Description	Purpose: Determination of inter- and intra-day variations in tear flow rate, tear fluid protein concentration as well as protein composition regarding their impact for future biomarker studies. Methods: Tear fluid was collected non-invasively from 18 healthy subjects performing Schirmer tests at four different time points repetitive in a period of two days. The tear flow rate with Schirmer test strips was measured. Proteins were extracted from strips and quantified using amino acid analysis. Protein composition was analyzed by data-independent (DIA) based mass spectrometry. To exclude any impairments to health, volunteers underwent a detailed neurological as well as an ophthalmological examination. Results: Whether tear fluid was collected from OS or OD did not affect the tear flow rate (p ≈ 0.63) or protein concentration (p ≈ 0.97) of individual subjects. Moreover, protein concentration was independent from the tear volume, so that a change in volume would only have an effect on total protein amount. When the examination days were compared, investigation of tear flow rate (p ≈ 0.001) and protein concentration (p ≈ 0.0003) indicated significant differences. Further mass spectrometric analysis of tear fluid revealed eleven differentially regulated proteins when comparing both examination days. Conclusion: Our findings provide evidence of inter-day variation in tear flow rate, tear proteome concentration and composition in healthy subjects, suggesting that inter-day variation need to be taken into consideration in biomarker research of tear fluid. Identified proteins were assigned to functions in the immune response, oxidative and reducing processes, as well as mannose metabolism.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:27.361.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annika Guntermann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-28 07:37:25	ID requested
1	2023-10-24 09:02:41	announced
⏵ 2	2023-11-14 09:06:30	announced	2023-11-14: Updated project metadata.

Guntermann A, Fatoba O, Kronenberg M, Reinehr S, Grotegut P, Schargus M, Tsai T, Ivanova S, Serschnitzki B, Kumowski N, Maier C, Marcus K, Dick HB, Joachim SC, May C, Investigation of Inter- and Intra-Day Variability of Tear Fluid Regarding Flow Rate, Protein Concentration as well as Protein Composition. Invest Ophthalmol Vis Sci, 64(13):13(2023) [pubmed]

ProteomeXchange project tag: Human Brain Proteome Project (HUPO_HBPP) (B/D-HPP)

submitter keyword: mass spectrometry,tear fluid, Schirmer test, tear flow rate

tear volume, protein concentration

Caroline May
contact affiliation	Functional Proteomics Medizinisches Proteom-Center Germany
contact email	Caroline.May@rub.de
lab head
Annika Guntermann
contact affiliation	Medizinisches Proteom-Center
contact email	Annika.Guntermann@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD037811

PRIDE project URI

• PRIDE
  1. PXD037811
     1. Label: PRIDE project
     2. Name: Investigation of inter- and intra-day variability of tear fluid regarding flow rate, protein concentration as well as protein composition