PXD037809

PXD037809 is an original dataset announced via ProteomeXchange.

Title	Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo
Description	Extracellular vesicles are membranous nanoparticles that convey signaling between cells, tissues and organs. By applying fluorescence tracing and SILAC-labeling paired with (phospho)proteomics, we identified the transfer of functional insulinotropic protein cargo via adipocyte-derived extracellular vesicles (AdEVs) from adipose tissue to pancreatic ß-cells in vivo and in vitro. AdEV-derived proteins were targets for phosphorylation, increased the overall abundances and phosphosite dynamics of insulinotropic GPCR/cAMP/PKA pathways and amplified 1st-phase glucose-stimulated insulin secretion (GSIS) in murine islets. Notably, insulinotropic effects were restricted to AdEVs from obese and insulin resistant, but not lean mice, which was consistent with differential protein loads and AdEV luminal morphologies. Pre-treatment with AdEVs from obese mice amplified insulin secretion and glucose tolerance in mice independent from hyperglycemia. These data suggest that secreted AdEVs can inform pancreatic ß-cells about adipose tissue insulin resistance in order to amplify GSIS in times of increased insulin demand.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:05.839.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalie Krahmer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-28 07:36:44	ID requested
1	2023-03-11 12:53:08	announced
⏵ 2	2023-11-14 08:44:09	announced	2023-11-14: Updated project metadata.

Kulaj K, Harger A, Bauer M, Caliskan Ö S, Gupta TK, Chiang DM, Milbank E, Reber J, Karlas A, Kotzbeck P, Sailer DN, Volta F, Lutter D, Prakash S, Merl-Pham J, Ntziachristos V, Hauner H, Pfaffl MW, Tsch, ö, p MH, M, ü, ller TD, Hauck SM, Engel BD, Gerdes JM, Pfluger PT, Krahmer N, Stemmer K, Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo. Nat Commun, 14(1):709(2023) [pubmed]

submitter keyword: proteomics, insulin secretion, SILAC,Extracellular vesicles, adipose tissue, phosphoproteomics, LC-MS/MS

Natalie Krahmer
contact affiliation	Emmy-Noether Research Group Cellular Proteomics and Metabolic Signaling Institute for Diabetes and Obesity Helmholtz Diabetes Center Germany
contact email	natalie.krahmer@helmholtz-muenchen.de
lab head
Natalie Krahmer
contact affiliation	Helmholtz Center Munich
contact email	natalie.krahmer@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037809

PRIDE project URI

• PRIDE
  1. PXD037809
     1. Label: PRIDE project
     2. Name: Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo