PXD037791

PXD037791 is an original dataset announced via ProteomeXchange.

Title	Surface protein profiling of prostate derived extracellular vesicles
Description	Extracellular vesicles (EVs) are mediators of intercellular communication and a promising class of biomarkers. Surface proteins of EV play decisive roles in establishing a connection with recipient cells, and they are putative targets in diagnostic assays. Analysis of the surface proteins can thus both illuminate the biological functions of EVs and help identify potential biomarkers. The surface proteins of intact seminal fluid ssmall EV (SF-sEVs and PC3) sEVs were labeled using sulfo-NHS-SS-biotin, a membrane-impermeable reagent reacting with primary amines. The fractions obtained from the purification process were: (i) protein in total sEV lysate (Total); (ii) proteins isolated from sEV surfaces (Surface), and (iii) supernatant proteins left after isolation of surface proteins (Total minus Surface). Proteins from each fraction were digested by trypsin and analyzed by HRMS. A total PC3 cell lysate was prepared andanalyzed as a control.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:34.706.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claudia Fredolini
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-28 02:27:03	ID requested
1	2023-03-11 10:47:28	announced
⏵ 2	2023-11-14 08:43:36	announced	2023-11-14: Updated project metadata.

Manouchehri Doulabi E, Fredolini C, Gallini R, L, ö, f L, Shen Q, Ikebuchi R, Dubois L, Azimi A, Loudig O, Gabrielsson S, Landegren U, Larsson A, Bergquist J, Kamali-Moghaddam M, Surface protein profiling of prostate-derived extracellular vesicles by mass spectrometry and proximity assays. Commun Biol, 5(1):1402(2022) [pubmed]

submitter keyword: Human, seminal fluid, cell supernatant

Masood Kamali-Moghaddam
contact affiliation	Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
contact email	masood.kamali@igp.uu.se
lab head
Claudia Fredolini
contact affiliation	KTH-Royal Institute of Technology, Protein Science Department Science for Life Laboratory
contact email	claudia.fredolini@scilifelab.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037791

PRIDE project URI

• PRIDE
  1. PXD037791
     1. Label: PRIDE project
     2. Name: Surface protein profiling of prostate derived extracellular vesicles