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PXD037781

PXD037781 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleModeling mammalian spermatogonial differentiation and meiotic initiation in vitro
DescriptionIn mammalian testes, premeiotic spermatogonia respond to retinoic acid by completing an essential lengthy differentiation program before initiating meiosis. The molecular and cellular changes directing these developmental processes remain largely undefined. This wide gap in knowledge is due to two critical unresolved technical challenges: (1) lack of robust and reliable in vitro models to study differentiation and meiotic initiation; and (2) lack of methods to isolate large and pure populations of male germ cells at each stage of differentiation and at meiotic initiation. Here, we report a facile in vitro differentiation and meiotic initiation system that can be readily manipulated, including the use of chemical agents that cannot be safely administered to live animals. In addition, we present a transgenic mouse model enabling fluorescence-activated cell sorting-based isolation of millions of spermatogonia at specific developmental stages as well as meiotic spermatocytes
HostingRepositoryjPOST
AnnounceDate2022-10-28
AnnouncementXMLSubmission_2022-10-27_13:07:42.277.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKelsey Fisher-Wellman
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-27 13:07:39ID requested
12022-10-27 13:07:42announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: sperm
Contact List
Geyer, Christopher
lab head
Kelsey Fisher-Wellman
contact affiliationEast Carolina Diabetes and Obesity Institute
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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