PXD037781
PXD037781 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro |
Description | In mammalian testes, premeiotic spermatogonia respond to retinoic acid by completing an essential lengthy differentiation program before initiating meiosis. The molecular and cellular changes directing these developmental processes remain largely undefined. This wide gap in knowledge is due to two critical unresolved technical challenges: (1) lack of robust and reliable in vitro models to study differentiation and meiotic initiation; and (2) lack of methods to isolate large and pure populations of male germ cells at each stage of differentiation and at meiotic initiation. Here, we report a facile in vitro differentiation and meiotic initiation system that can be readily manipulated, including the use of chemical agents that cannot be safely administered to live animals. In addition, we present a transgenic mouse model enabling fluorescence-activated cell sorting-based isolation of millions of spermatogonia at specific developmental stages as well as meiotic spermatocytes |
HostingRepository | jPOST |
AnnounceDate | 2022-10-28 |
AnnouncementXML | Submission_2022-10-27_13:07:42.277.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kelsey Fisher-Wellman |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-10-27 13:07:39 | ID requested | |
⏵ 1 | 2022-10-27 13:07:42 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: sperm |
Contact List
Geyer, Christopher | |
---|---|
lab head | |
Kelsey Fisher-Wellman | |
contact affiliation | East Carolina Diabetes and Obesity Institute |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
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