PXD037753
PXD037753 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A multi-omics analysis of glioma chemoresistance using a hybrid microphysiological model of glioblastoma |
Description | Chemoresistance is a major clinical challenge in management of glioblastoma (GBM) Temozolomide (TMZ) is the chemotherapeutic drug of choice for GBM; however, the therapeutic effect of TMZ is limited due to the development of resistance. Recapitulating GBM chemoresistance in a controlled environment is thus essential in understanding the mechanism of chemoresistance. Herein, we present a hybrid microphysiological model of chemoresistant GBM-on-a-chip (HGoC) by directly co-culturing TMZ-resistant GBM spheroids with healthy neurons to mimic the microenvironment of both the tumor and the surrounding healthy tissue. We characterized the model with proteomics, lipidomics, and secretome assays. The results showed that our artificial model recapitulated the molecular signatures of recurrent GBM in humans. Both showed alterations in vesicular transport and cholesterol pathways, mitotic quiescence, and a switch in metabolism to oxidative phosphorylation associated with a transition from mesenchymal to amoeboid. This is the first report to unravel the interplay of all these molecular changes as a mechanism of chemoresistance in glioblastoma. Moreover, we have shown that acquisition of resistance increases invasiveness and the presence of neurons decreases this property. 1. Seyfoori, A., et al., Self-filling microwell arrays (SFMAs) for tumor spheroid formation. Lab Chip, 2018. 18(22): p. 3516-3528. 2. Amereh, M., et al., In-Silico Modeling of Tumor Spheroid Formation and Growth. Micromachines (Basel), 2021. 12(7). 3. Senko, M.W., et al., Novel Parallelized Quadrupole/Linear Ion Trap/Orbitrap Tribrid Mass Spectrometer Improving Proteome Coverage and Peptide Identification Rates. Analytical Chemistry, 2013. 85(24): p. 11710-11714. 4. Zhou, G., et al., NetworkAnalyst 3.0: a visual analytics platform for comprehensive gene expression profiling and meta-analysis. Nucleic Acids Res, 2019. 47(W1): p. W234-w241. 5. Xia, J., E.E. Gill, and R.E. Hancock, NetworkAnalyst for statistical, visual and network-based meta-analysis of gene expression data. Nat Protoc, 2015. 10(6): p. 823-44. 6. Breuer, K., et al., InnateDB: systems biology of innate immunity and beyond--recent updates and continuing curation. Nucleic Acids Res, 2013. 41(Database issue): p. D1228-33. |
HostingRepository | PRIDE |
AnnounceDate | 2025-06-09 |
AnnouncementXML | Submission_2025-06-08_16:06:50.663.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rui Vitorino |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-10-27 02:35:49 | ID requested | |
⏵ 1 | 2025-06-08 16:06:51 | announced |
Publication List
10.1002/advs.202412505; |
Amereh M, Seyfoori A, Shojaei S, Lane S, Zhao T, Shokrollahi Barough M, Lum JJ, Walter P, Akbari M, Tumoroid Model Reveals Synergistic Impairment of Metabolism by Iron Chelators and Temozolomide in Chemo-Resistant Patient-derived Glioblastoma Cells. Adv Sci (Weinh), 12(20):e2412505(2025) [pubmed] |
Keyword List
submitter keyword: A multi-omics analysis of glioma chemoresistance using a hybrid microphysiological model of glioblastoma |
Contact List
Mohsen Akbari | |
---|---|
contact affiliation | University of Victoria, Department of Mechanical Engineering, PO Box 1700 STN CSC, Victoria, BC, V8W 2Y2, Canada |
contact email | Shahla.Shojaei@umanitoba.ca |
lab head | |
Rui Vitorino | |
contact affiliation | Universidade de Aveiro |
contact email | rvitorino@ua.pt |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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