PXD037751 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An Optimized Suspension Trapping (S-Trap) Method for Phosphoproteomics Sample Preparation |
Description | The performance of the S-Trap protocol for phosphoproteomics studies for proteome and phosphoproteome was systematically evaluated in big-scale and small-scale samples. The results showed the existing protocol was problematic for phsophopeptides enrichment. After employing optimized S-Trap methodology, more peptides and phosphopeptides are found in the application for extracellular vesicles. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:48:48.252.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fujia Wang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-27 02:26:11 | ID requested | |
1 | 2023-06-19 06:27:13 | announced | |
2 | 2023-11-14 09:01:25 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 05:48:49 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1021/acs.analchem.3c00324; |
Wang F, Veth T, Kuipers M, Altelaar M, Stecker KE, Optimized Suspension Trapping Method for Phosphoproteomics Sample Preparation. Anal Chem, 95(25):9471-9479(2023) [pubmed] |
Keyword List
submitter keyword: Phosphoproteomics |
sample preparation |
method optimization |
Contact List
Maarten Altelaar |
contact affiliation | Biomolecular Mass Spectrometry and Proteomics group, Utrecht University, The Netherlands |
contact email | m.altelaar@uu.nl |
lab head | |
Fujia Wang |
contact affiliation | Utrecht University |
contact email | f.wang3@uu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD037751 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037751
- Label: PRIDE project
- Name: An Optimized Suspension Trapping (S-Trap) Method for Phosphoproteomics Sample Preparation