PXD037731 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Isolation of interstitial fluids reveals novel secreted bioactive proteins from muscle and fat tissues |
Description | Proteins are secreted from various cells to send information to neighboring cells or distant tissues. Because of the highly integrated nature of energy balance systems, there has been a great deal of interest in myokines and adipokines, proteins secreted from muscle and fat tissues, respectively. These have been challenging to study through proteomics because serum is loaded with super-abundant proteins that limit the detection of proteins that have a low, hormone-like abundance. We show here that interstitial fluids (IFs) can be harvested easily from muscle and fat tissues of mice by very low-speed centrifugation and these fluids show a very different protein constellation than that of plasma or tissues. Under several different perturbations in vivo, like exercise or cold, quantitative Mass Spectrometry of these IFs allowed for the identification of many novel myokines and adipokines, including factors both increased and decreased in abundance. Finally, we identify prosaposin, a well-known neurotrophic factor, as a secreted product of both muscle and fat tissues; recombinant prosaposin stimulates a gene program related to thermogenesis in primary fat cells, indicating one potential function for PSAP. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:38:34.060.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jonathan Van Vranken |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-26 01:20:41 | ID requested | |
1 | 2023-02-10 08:37:41 | announced | |
⏵ 2 | 2023-11-14 07:38:34 | announced | 2023-11-14: Updated project metadata. |
Publication List
Mittenb, ΓΌ, hler MJ, Jedrychowski MP, Van Vranken JG, Sprenger HG, Wilensky S, Dumesic PA, Sun Y, Tartaglia A, Bogoslavski D, A M, Xiao H, Blackmore KA, Reddy A, Gygi SP, Chouchani ET, Spiegelman BM, Isolation of extracellular fluids reveals novel secreted bioactive proteins from muscle and fat tissues. Cell Metab, 35(3):535-549.e7(2023) [pubmed] |
Keyword List
submitter keyword: brown fat,Interstial fluid, muscle |
Contact List
Bruce Spiegelman |
contact affiliation | Dana-Farber Cancer Institute and Harvard Medical School |
contact email | bruce_spiegelman@dfci.harvard.edu |
lab head | |
Jonathan Van Vranken |
contact affiliation | Harvard Medical School |
contact email | van@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037731
- Label: PRIDE project
- Name: Isolation of interstitial fluids reveals novel secreted bioactive proteins from muscle and fat tissues