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PXD037722

PXD037722 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Novel Approach to Interrogating the Effects of Chemical Warfare Agent Exposure Using Organ-on-a-Chip Technology and Multiomic Analysis
DescriptionOrgan-on-a-chip platforms are utilized in global bioanalytical and toxicological studies as a way to reduce materials and increase throughput as compared to in vivo based experiments. These platforms bridge the infrastructure and regulatory gaps between in vivo animal work and human systems, with models that exemplify active biological pathways. In conjunction with the advent of increased capabilities associated with next generation sequencing and mass spectrometry based ‘-omic’ technologies, organ-on-a-chip platforms provide an excellent opportunity to investigate the global changes at multiple biological levels, including the transcriptome, proteome and metabolome. When investigated concurrently, a complete profile of cellular and regulatory perturbations can be characterized following treatment with specific agonists. In this study, global effects were observed and analyzed following liver chip exposure to the chemical warfare agent, VX. Even though the primary mechanism of action of VX (i.e. acetylcholinesterase inhibition) is well characterized, recent in vivo studies suggest additional protein binding partners that are implicated in metabolism and cellular energetic pathways. Also, secondary toxicity associated with peripheral organ systems, especially in human tissues, is not well defined. Our results demonstrate the efficacy of utilizing an organ-on-a-chip platform as a surrogate system to traditional in vivo studies. This is realized by specifically indicating significant dysregulation of a number of cellular processes in response to VX exposure including but not limited to amino acid synthesis, drug metabolism, and energetics pathways.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:28:25.608.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterConor Jenkins
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-26 00:58:11ID requested
12023-03-10 18:35:31announced
22023-11-14 08:28:26announced2023-11-14: Updated project metadata.
Publication List
Goralski TDP, Jenkins CC, Angelini DJ, Horsmon JR, Dhummakupt ES, Rizzo GM, Simmons BL, Liem AT, Roth PA, Karavis MA, Hill JM, Sekowski JW, Glover KP, A novel approach to interrogating the effects of chemical warfare agent exposure using organ-on-a-chip technology and multiomic analysis. PLoS One, 18(2):e0280883(2023) [pubmed]
Keyword List
submitter keyword: multiomics, organ on a chip,Liver, lcms, organophosphate
Contact List
Conor C Jenkins
contact affiliationU.S. Army DEVCOM CBC
contact emailconor.c.jenkins.civ@army.mil
lab head
Conor Jenkins
contact affiliationUS Army DEVCOM CBC
contact emailconor.c.jenkins.civ@army.mil
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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