PXD037722 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Novel Approach to Interrogating the Effects of Chemical Warfare Agent Exposure Using Organ-on-a-Chip Technology and Multiomic Analysis |
Description | Organ-on-a-chip platforms are utilized in global bioanalytical and toxicological studies as a way to reduce materials and increase throughput as compared to in vivo based experiments. These platforms bridge the infrastructure and regulatory gaps between in vivo animal work and human systems, with models that exemplify active biological pathways. In conjunction with the advent of increased capabilities associated with next generation sequencing and mass spectrometry based ‘-omic’ technologies, organ-on-a-chip platforms provide an excellent opportunity to investigate the global changes at multiple biological levels, including the transcriptome, proteome and metabolome. When investigated concurrently, a complete profile of cellular and regulatory perturbations can be characterized following treatment with specific agonists. In this study, global effects were observed and analyzed following liver chip exposure to the chemical warfare agent, VX. Even though the primary mechanism of action of VX (i.e. acetylcholinesterase inhibition) is well characterized, recent in vivo studies suggest additional protein binding partners that are implicated in metabolism and cellular energetic pathways. Also, secondary toxicity associated with peripheral organ systems, especially in human tissues, is not well defined. Our results demonstrate the efficacy of utilizing an organ-on-a-chip platform as a surrogate system to traditional in vivo studies. This is realized by specifically indicating significant dysregulation of a number of cellular processes in response to VX exposure including but not limited to amino acid synthesis, drug metabolism, and energetics pathways. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:28:25.608.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Conor Jenkins |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-26 00:58:11 | ID requested | |
1 | 2023-03-10 18:35:31 | announced | |
⏵ 2 | 2023-11-14 08:28:26 | announced | 2023-11-14: Updated project metadata. |
Publication List
Goralski TDP, Jenkins CC, Angelini DJ, Horsmon JR, Dhummakupt ES, Rizzo GM, Simmons BL, Liem AT, Roth PA, Karavis MA, Hill JM, Sekowski JW, Glover KP, A novel approach to interrogating the effects of chemical warfare agent exposure using organ-on-a-chip technology and multiomic analysis. PLoS One, 18(2):e0280883(2023) [pubmed] |
Keyword List
submitter keyword: multiomics, organ on a chip,Liver, lcms, organophosphate |
Contact List
Conor C Jenkins |
contact affiliation | U.S. Army DEVCOM CBC |
contact email | conor.c.jenkins.civ@army.mil |
lab head | |
Conor Jenkins |
contact affiliation | US Army DEVCOM CBC |
contact email | conor.c.jenkins.civ@army.mil |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037722 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037722
- Label: PRIDE project
- Name: A Novel Approach to Interrogating the Effects of Chemical Warfare Agent Exposure Using Organ-on-a-Chip Technology and Multiomic Analysis