PXD037715

PXD037715 is an original dataset announced via ProteomeXchange.

Title	Comparison of milk fat globule membrane protein profile among bovine, goat and camel milk based on label free proteomic techniques
Description	Milk fat globule membrane (MFGM) proteins, which are recognized to play a variety of biological roles; nevertheless, their composition and interspecies complexity remain unknown. This study aimed to identify and quantify proteins extracted from MFGM-enriched fractions of bovine, goat and camel milk by a label free proteomics approach. We identified 1579 proteins, substantially increasing the number of MFGM proteins identified for these species. The results of functional analysis showed that the identified MFGM proteins had similar functional annotations, with cellular processes, intracellular anatomical structures and binding being the predominant GO annotations. And most of the proteins were involved in KEGG pathways such as protein processing of ribosomes and endoplasmic reticulum. Subsequently, multiple statistical methods were used to compare the differences in MFGM protein quantification among different species. The results of principal component analysis showed that bovine, goat and camel milk MFGM proteins were significantly different, among which CSN1S1, XDH, LTF and PI4KA played an important role in taxonomic identification. Meanwhile the results of hierarchical clustering showed that the differences (p<0.05) between bovine and goat milk were smaller than those of camel milk. These new data deepen the understanding of the protein composition of MFGM and its possible physiological roles, providing directions for the production of specific functional milk proteins.
HostingRepository	iProX
AnnounceDate	2022-10-24
AnnouncementXML	Submission_2023-12-06_19:09:36.613.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Binsong Han
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913; scientific name: Capra hircus; NCBI TaxID: 9925; scientific name: Camelus bactrianus; NCBI TaxID: 9837;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-26 00:09:09	ID requested
1	2022-11-30 19:01:43	announced
⏵ 2	2023-12-06 19:09:37	announced	2023-12-07: Update information.

Han B, Zhang L, Zhou P, Comparison of milk fat globule membrane protein profile among bovine, goat and camel milk based on label free proteomic techniques. Food Res Int, 162(Pt B):112097(2022) [pubmed]

submitter keyword: proteomics, MFGM protein, principal component analysis, different mammals

Peng Zhou
contact affiliation	State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
contact email	zhoupeng@jiangnan.edu.cn
lab head
Binsong Han
contact affiliation	State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
contact email	hanbinsong@live.com
dataset submitter

iProX dataset URI