PXD037707

PXD037707 is an original dataset announced via ProteomeXchange.

Title	Depletion of melanin exposes Aspergillus flavus conidial surface proteins and alters the surface architecture and virulence.
Description	A. flavus keratitis is one of the predominant fungal infections affecting the tropical parts of the world. Melanin is a virulence factor in numerous pathogenic fungi and the melanin layer maintains the cell wall structure and provides chemical and physical protection to the organism. However, the molecular and biological mechanisms modulating melanin-mediated host-pathogen interaction in A. flavus keratitis are not well understood. This work aimed to compare the morphology, surface proteome profile and virulence of melanized and non-melanized conidia of A. flavus. An environmental isolate and two clinical isolates were included in this study. L-DOPA melanin pathway-specific inhibitor kojic acid (KA) as used to prepare non-melanized conidia. Conidial surface proteins were extracted using 100% formic acid at 0 ºC and the extracted proteins were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE). Formic acid extracts were analyzed in an Orbitrap Velos pro-mass spectrometer. Conidial surface morphology difference was examined using scanning electron microscopy (SEM). Furthermore, lactophenol and calcofluor staining were performed to check the morphology and staining efficiency of melanized conidia (MC) and non-melanized conidia (NMC). A Galleria mellonella insect model was used to examine the virulence efficiency of MC and NMC. Kojic acid treatment inhibited melanin synthesis in A. flavus and the conidial surface protein profile was significantly different in kojic acid-treated non-melanized conidia. Several cell wall-associated proteins and proteins responsible for oxidative stress, carbohydrate, and chitin metabolic pathway were found only in the formic acid extracts of NMC. SEM analysis showed the conidial surface morphology difference between the NMC and MC indicating the role of melanin in the structural integrity of the conidial cell wall. The levels of calcofluor staining efficiency were different, but there was no microscopic morphology difference in lactophenol cotton blue staining of MC and NMC. Evaluation of virulence of MC and NMC in G. mellonella model showed NMC was less virulent compared to MC . Our results showed that the integrity of the conidial surface is controlled by the melanin layer. The alteration in the surface protein profile indicated that many surface proteins are masked by the melanin layer and hence melanin can modulate the host response by preventing the exposure of fungal proteins to the host immune defense system. G. mellonella in vivo virulence assay also confirmed that the non-melanized conidia were susceptible to host defense as in other Aspergillus pathogens.
HostingRepository	PRIDE
AnnounceDate	2024-05-23
AnnouncementXML	Submission_2024-05-23_00:57:59.278.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rudhra Ondipilli
SpeciesList	scientific name: Aspergillus flavus FRR 3643; NCBI TaxID: 1431647;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-25 04:44:26	ID requested
⏵ 1	2024-05-23 00:58:00	announced

10.1007/s00253-024-13107-4;

Rudhra O, Gnanam H, Sivaperumal S, Namperumalsamy V, Prajna L, Kuppamuthu D, Melanin depletion affects Aspergillus flavus conidial surface proteins, architecture, and virulence. Appl Microbiol Biotechnol, 108(1):291(2024) [pubmed]

submitter keyword: Non-melanized conidia, Melanized conidia,Melanin, and Surface protein

Kuppamuthu Dharmalingam
contact affiliation	DR.G.VENKATASAMY RESEARCH INSTITUTE, Aravind medical research foundation, MADURAI
contact email	dharmalingam.k@gmail.com
lab head
Rudhra Ondipilli
contact affiliation	Aravind Medical research foundation
contact email	asharuthra@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD037707

PRIDE project URI

• PRIDE
  1. PXD037707
     1. Label: PRIDE project
     2. Name: Depletion of melanin exposes Aspergillus flavus conidial surface proteins and alters the surface architecture and virulence.