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PXD037706

PXD037706 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMembrane Protein Modification Modulates Big and Small Extracellular Vesicle Biodistribution and Tumorigenic Potential in Breast Cancers in vivo
DescriptionExtracellular vesicles (EVs) are released by cells to mediate intercellular communication under pathological and physiological conditions. While small EVs (sEVs; <100–200 nm, exosomes) are intensely investigated, the properties and functions of medium and large EVs (big EVs [bEVs]; >200 nm, microvesicles) are less well explored. Here, we identify bEVs and sEVs as distinct EV populations, and determine that bEVs are released in a greater bEV:sEV ratio in the aggressive human triple-negative breast cancer (TNBC) subtype. PalmGRET, bioluminescence resonance energy transfer (BRET)-based EV reporter, reveals dose-dependent EV biodistribution at non-lethal and physiological EV dosages, as compared to lipophilic fluorescent dyes. The bEVs and sEVs exhibit unique biodistribution profiles, yet individually promote in vivo tumor growth in a syngeneic immunocompetent TNBC breast tumor murine model. The bEVs and sEVs share mass spectrometry (MS)-identified tumor progression-associated EV surface membrane proteins (tpEVSurfMEMs), which include SLC29A1, CD9 and CD44. Remarkably, tpEVSurfMEM depletion attenuates EV lung organotropism, alters biodistribution, and reduces protumorigenic potential. This study identifies distinct in vivo property and function of bEVs and sEVs in breast cancer, which suggest the significant role of bEVs in diseases, diagnostic and therapeutic applications.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:17:00.767.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBryan John Magoling
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-25 04:44:07ID requested
12023-12-08 08:13:40announced
22024-10-22 06:17:01announced2024-10-22: Updated project metadata.
Publication List
10.1002/adma.202208966;
Magoling BJA, Wu AY, Chen YJ, Wong WW, Chuo ST, Huang HC, Sung YC, Hsieh HT, Huang P, Lee KZ, Huang KW, Chen RH, Chen Y, Lai CP, Membrane Protein Modification Modulates Big and Small Extracellular Vesicle Biodistribution and Tumorigenic Potential in Breast Cancers In Vivo. Adv Mater, 35(13):e2208966(2023) [pubmed]
Keyword List
ProteomeXchange project tag: Cancer (B/D-HPP)
submitter keyword: LC-MSMS,Mouse, Extracellular vesicle
Contact List
Charles P. Lai
contact affiliationInstitute of Atomic and Molecular Sciences, Academia Sinica, Taiwan
contact emaillaicharles@sinica.edu.tw
lab head
Bryan John Magoling
contact affiliationInstitute of Atomic and Molecular Sciences, Academia Sinica
contact emailbryanmagoling@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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