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PXD037700

PXD037700 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics analysis of lung tissue from E18.5 embryos with mesenchymal deletion of VLK
DescriptionVertebrate lonesome kinase (VLK) is the only known secreted tyrosine kinase and responsible for the phosphorylation of a broad range of secretory pathway-resident and extracellular matrix proteins. However, its cell-type specific functions in vivo are still largely unknown. Therefore, we generated mice with a mesenchyme-specific knockout of the VLK gene (protein kinase domain containing, cytoplasmic (Pkdcc)). Most of the homozygous mice die shortly after birth, most likely as a consequence of their lung abnormalities and consequent respiratory failure. E18.5 embryonic lungs showed a reduction of alveolar type II cells, smaller bronchi, and an increased lung tissue density. Global mass spectrometry-based quantitative proteomics identified 112 proteins with significantly and at least 1.5fold differential abundance between genotypes. Twenty-five of these had been assigned to the extracellular region and 15 to the mouse matrisome. Specifically, fibromodulin and matrilin 4, which are involved in extracellular matrix organization, were significantly more abundant in lungs from Pkdcc knockout embryos. These results support a role for mesenchyme-derived VLK in lung development through regulation of matrix dynamics and the resulting non-cell-autonomous modulation of alveolar epithelial cell differentiation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:44:48.647.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD037700
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterUlrich auf dem Keller
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-25 04:38:39ID requested
12023-04-11 06:21:19announced
22023-11-14 08:44:49announced2023-11-14: Updated project metadata.
Publication List
Br, ü, tsch SM, Madzharova E, Pantasis S, W, ü, stemann T, Gurri S, Steenbock H, Gazdhar A, Kuhn G, Angel P, Bellusci S, Brinckmann J, Auf dem Keller U, Werner S, Bordoli MR, Mesenchyme-derived vertebrate lonesome kinase controls lung organogenesis by altering the matrisome. Cell Mol Life Sci, 80(4):89(2023) [pubmed]
10.6019/PXD037700;
Keyword List
submitter keyword: embryo, lung,vertrebrate lonesome kinase
Contact List
Ulrich auf dem Keller
contact affiliationDepartment of Biotechnology and Biomedicine, Technical University of Denmark
contact emailuadk@dtu.dk
lab head
Ulrich auf dem Keller
contact affiliationTechnical University of Denmark
contact emailulrich.aufdemkeller@cell.biol.ethz.ch
dataset submitter
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