PXD037700

PXD037700 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics analysis of lung tissue from E18.5 embryos with mesenchymal deletion of VLK
Description	Vertebrate lonesome kinase (VLK) is the only known secreted tyrosine kinase and responsible for the phosphorylation of a broad range of secretory pathway-resident and extracellular matrix proteins. However, its cell-type specific functions in vivo are still largely unknown. Therefore, we generated mice with a mesenchyme-specific knockout of the VLK gene (protein kinase domain containing, cytoplasmic (Pkdcc)). Most of the homozygous mice die shortly after birth, most likely as a consequence of their lung abnormalities and consequent respiratory failure. E18.5 embryonic lungs showed a reduction of alveolar type II cells, smaller bronchi, and an increased lung tissue density. Global mass spectrometry-based quantitative proteomics identified 112 proteins with significantly and at least 1.5fold differential abundance between genotypes. Twenty-five of these had been assigned to the extracellular region and 15 to the mouse matrisome. Specifically, fibromodulin and matrilin 4, which are involved in extracellular matrix organization, were significantly more abundant in lungs from Pkdcc knockout embryos. These results support a role for mesenchyme-derived VLK in lung development through regulation of matrix dynamics and the resulting non-cell-autonomous modulation of alveolar epithelial cell differentiation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:48.647.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037700
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ulrich auf dem Keller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-25 04:38:39	ID requested
1	2023-04-11 06:21:19	announced
⏵ 2	2023-11-14 08:44:49	announced	2023-11-14: Updated project metadata.

Br, ü, tsch SM, Madzharova E, Pantasis S, W, ü, stemann T, Gurri S, Steenbock H, Gazdhar A, Kuhn G, Angel P, Bellusci S, Brinckmann J, Auf dem Keller U, Werner S, Bordoli MR, Mesenchyme-derived vertebrate lonesome kinase controls lung organogenesis by altering the matrisome. Cell Mol Life Sci, 80(4):89(2023) [pubmed]

10.6019/PXD037700;

submitter keyword: embryo, lung,vertrebrate lonesome kinase

Ulrich auf dem Keller
contact affiliation	Department of Biotechnology and Biomedicine, Technical University of Denmark
contact email	uadk@dtu.dk
lab head
Ulrich auf dem Keller
contact affiliation	Technical University of Denmark
contact email	ulrich.aufdemkeller@cell.biol.ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037700
     1. Label: PRIDE project
     2. Name: Quantitative proteomics analysis of lung tissue from E18.5 embryos with mesenchymal deletion of VLK