PXD037697 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FAM69C functions as a kinase for eIF2α and promotes stress granule assembly |
Description | Stress granules are dynamic cytoplasmic ribonucleoprotein granules that assemble in response to cellular stress. Aberrant formation of stress granules has been linked to neurodegenerative diseases. However, the molecular mechanisms underlying the initiation of stress granules remain elusive. Here we report that the brain-enriched protein kinase FAM69C promotes stress granule assembly through phosphorylation of eukaryotic translation initiation factor 2 (eIF2α). FAM69C physically interacts with eIF2α and functions as a stress-specific kinase for eIF2α, leading to stress-induced protein translation arrest and stress granule assembly. Primary microglia derived from Fam69c knockout mice exhibit aberrant stress granule assembly in response to oxidative stress and ATP. Defective stress granule assembly in microglia correlates with the formation of ASC specks and NLRP3 inflammasome activation, whereas induction of stress granule precludes inflammasome formation. Consistently, increased NLRP3 levels, caspase-1 cleavage and Il18 expression corroborate microglia-associated neuroinflammation in aged Fam69c knockout mice. Our study demonstrates that FAM69C is critical for stress granule assembly and suggests its role in the regulation of microglia function. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_06:49:28.856.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fan Mei |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-25 04:31:59 | ID requested | |
1 | 2023-04-21 05:28:03 | announced | |
⏵ 2 | 2023-11-14 06:49:29 | announced | 2023-11-14: Updated project metadata. |
Publication List
Wu Z, Mei F, Gan Y, Liu A, Hu J, Jin Y, Yin Y, and promotes stress granule assembly. EMBO Rep, 24(5):e55641(2023) [pubmed] |
Keyword List
submitter keyword: eIF2α |
FAM69C |
inflammasome |
stress response |
stress granule. |
Contact List
Fan Mei |
contact affiliation | Institute of Systems Biomedicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China |
contact email | meifan@hsc.pku.edu.cn |
lab head | |
Fan Mei |
contact affiliation | Peking University |
contact email | meifan@hsc.pku.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037697
- Label: PRIDE project
- Name: FAM69C functions as a kinase for eIF2α and promotes stress granule assembly