PXD037694

PXD037694 is an original dataset announced via ProteomeXchange.

Title	Proximity ligation mapping of microcephaly associated SMPD4 shows association with components of the nuclear pore mem-brane
Description	SMPD4 is a neutral sphingomylinase implicated in a specific type of congenital microcephaly. Although not intensively studied, SMPD4 deficiency has also been found to cause cell division defects. This suggests a role for SMPD4 in cell cycle and differentiation. In order to explore this role we used proximity ligation to identify the partners of SMPD4 in vivo in HEK293T cells. We found that these partners localize near the endoplasmic reticulum ( ER) and the nuclear membrane. Using mass spectrometry we could identify these partners and discovered that SMPD4 is closely asso-ciated with several nucleoporins, including NUP35, a nucleoporin directly involved in pore membrane curvature and pore insertion. This suggests that SMPD4 may play a role in this process.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:45:41.067.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeroen Demmers
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-25 03:50:50	ID requested
1	2022-10-25 09:55:29	announced
⏵ 2	2023-11-14 07:45:41	announced	2023-11-14: Updated project metadata.

Pi, ë, t ACA, Post M, Dekkers D, Demmers JAA, Fornerod M, Proximity Ligation Mapping of Microcephaly Associated SMPD4 Shows Association with Components of the Nuclear Pore Membrane. Cells, 11(4):(2022) [pubmed]

submitter keyword: Nuclear pore membrane

Sphingomyelinase

NUP35

SMPD4

n-smnase-3

Jeroen Demmers
contact affiliation	Protemics Center, Erasmus MC Rotterdam
contact email	j.demmers@erasmusmc.nl
lab head
Jeroen Demmers
contact affiliation	Proteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands
contact email	j.demmers@erasmusmc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD037694

PRIDE project URI

• PRIDE
  1. PXD037694
     1. Label: PRIDE project
     2. Name: Proximity ligation mapping of microcephaly associated SMPD4 shows association with components of the nuclear pore mem-brane