PXD037694 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proximity ligation mapping of microcephaly associated SMPD4 shows association with components of the nuclear pore mem-brane |
Description | SMPD4 is a neutral sphingomylinase implicated in a specific type of congenital microcephaly. Although not intensively studied, SMPD4 deficiency has also been found to cause cell division defects. This suggests a role for SMPD4 in cell cycle and differentiation. In order to explore this role we used proximity ligation to identify the partners of SMPD4 in vivo in HEK293T cells. We found that these partners localize near the endoplasmic reticulum ( ER) and the nuclear membrane. Using mass spectrometry we could identify these partners and discovered that SMPD4 is closely asso-ciated with several nucleoporins, including NUP35, a nucleoporin directly involved in pore membrane curvature and pore insertion. This suggests that SMPD4 may play a role in this process. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:45:41.067.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jeroen Demmers |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-25 03:50:50 | ID requested | |
1 | 2022-10-25 09:55:29 | announced | |
⏵ 2 | 2023-11-14 07:45:41 | announced | 2023-11-14: Updated project metadata. |
Publication List
Pi, ë, t ACA, Post M, Dekkers D, Demmers JAA, Fornerod M, Proximity Ligation Mapping of Microcephaly Associated SMPD4 Shows Association with Components of the Nuclear Pore Membrane. Cells, 11(4):(2022) [pubmed] |
Keyword List
submitter keyword: Nuclear pore membrane |
Sphingomyelinase |
NUP35 |
SMPD4 |
n-smnase-3 |
Contact List
Jeroen Demmers |
contact affiliation | Protemics Center, Erasmus MC Rotterdam |
contact email | j.demmers@erasmusmc.nl |
lab head | |
Jeroen Demmers |
contact affiliation | Proteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands |
contact email | j.demmers@erasmusmc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037694
- Label: PRIDE project
- Name: Proximity ligation mapping of microcephaly associated SMPD4 shows association with components of the nuclear pore mem-brane