PXD037693

PXD037693 is an original dataset announced via ProteomeXchange.

Title	Targeted protein of USP20 by LC-MS/MS in HepG2 cells
Description	Obesity is a metabolic disease accompanied by lipid metabolism that can cause hyperlipidemia, non-alcoholic fatty liver disease, and artery atherosclerosis. Sleeve gastrectomy (SG) is a type of bariatric surgery that can effectively treat obesity and improve lipid metabolism. However, its specific underlying mechanism remains unclear. Therefore, we herein performed SG, and sham surgery on two groups of diet-induced obese mice. The results showed that compared to the sham group, the SG group displayed a downregulation of deubiquitinase ubiquitin-specific peptidase 20 (USP20). And USP20 could promote lipid accumulation in vitro. Co-immunoprecipitation and mass spectrometry analyses showed that heat-shock protein family A member 2 (HSPA2) potentially acts as a substrate of USP20. HSPA2 was also downregulated in the SG group and could promote lipid accumulation in vitro. Further research showed that USP20 targeted and stabilized HSPA2 via the ubiquitin-proteasome pathway. The downregulation of the USP20-HSPA2 axis in diet-induced obese mice following SG improved lipid dysmetabolism, indicating that USP20-HSPA2 axis was a noninvasive therapeutic treatment to be investigated in the future.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:35.018.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenjie Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-25 03:49:56	ID requested
1	2022-12-22 10:14:38	announced
⏵ 2	2023-11-14 08:58:35	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: HepG2, LC-MS/MS, USP20

Zhang Wenjie
contact affiliation	Cheeloo College of Medicine, Shandong University
contact email	zhangwenjie18366118551@outlook.com
lab head
Wenjie Zhang
contact affiliation	Cheeloo College of Medicine, Shandong University, Jinan, China
contact email	zhangwenjie18366118551@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD037693

PRIDE project URI

• PRIDE
  1. PXD037693
     1. Label: PRIDE project
     2. Name: Targeted protein of USP20 by LC-MS/MS in HepG2 cells