PXD037675

PXD037675 is an original dataset announced via ProteomeXchange.

Title	The massive solubility changes of neuronal proteins upon simulated traumatic brain injury
Description	The molecular consequences of traumatic brain injuries or TBI are poorly understood. Here, we simulated TBI using an innovative laboratory apparatus employing a 5.1 kg dummy impact generating a ≤3,300 g-force acceleration and performed system-wide profiling of neuronal cells using Proteome Integral Solubility Alteration (PISA) assay. Dynamic impact leads to both reduction in neuron viability and massive solubility changes in the proteome. The affected proteins map not only to pathways such as cell adhesion, collagen and laminin structures, as well as response to stress, but also to other dense protein networks, such as immune response, complement and coagulation cascades. An energy absorbing material largely rescues the loss of cell viability and dampens proteome solubility changes. The cellular effects are found to be mainly due to the shockwave rather than the g-force acceleration. This study paves the way to introducing a proteome-based shock-meter as well as identifying TBI protein biomarkers and potential drug targets for prevention and intervention.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:49:30.979.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amir Ata Saei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-24 01:58:50	ID requested
1	2023-09-23 05:11:54	announced
⏵ 2	2023-11-14 07:49:31	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: PISA, proteomics, neurons, shock, TBI, dynamic impact, stability

Prof. Roman A. Zubarev
contact affiliation	Division of Physiological Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 65 Stockholm, Sweden
contact email	Roman.zubarev@ki.se
lab head
Amir Ata Saei
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact email	amirata.saei.dibavar@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD037675

PRIDE project URI

• PRIDE
  1. PXD037675
     1. Label: PRIDE project
     2. Name: The massive solubility changes of neuronal proteins upon simulated traumatic brain injury