PXD037659

PXD037659 is an original dataset announced via ProteomeXchange.

Title	Effect of knockdEffect of knockdown of mitochondrial atp1 mRNA by a custom-designed PPR protein on protein expression in Arabidopsis thaliana own of mitochondrial atp1 mRNA by a custom-designed PPR protein on protein expression in Arabidopsis thaliana
Description	We show that a custom-designed RNA-binding protein binds and specifically induces cleavage of atp1 RNA in mitochondria, significantly decreasing the abundance of the Atp1 protein and the assembled F1Fo ATP synthase in Arabidopsis thaliana. Changes to the proteome as a consequence of the modification were investigated by shotgun proteomics on enriched mitochondrial fractions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:23.057.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elke Stroeher
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-24 01:04:06	ID requested
1	2023-10-24 11:18:31	announced
⏵ 2	2023-11-14 09:07:30	announced	2023-11-14: Updated project metadata.

Vincis Pereira Sanglard L, Small ID, Colas des Francs-Small C, Using a Custom-Made Library of Pentatricopeptide Repeat Proteins. Int J Mol Sci, 24(17):(2023) [pubmed]

submitter keyword: ATP synthase, atp1, RNA processing factor 2 (RPF2),mitochondria, protein design, pentatricopeptide repeat (PPR)

Ian Small
contact affiliation	Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, Crawley, WA 6009, Australia
contact email	ian.small@uwa.edu.au
lab head
Elke Stroeher
contact affiliation	The University of Western Australia
contact email	elke.stroeher@uwa.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD037659

PRIDE project URI

• PRIDE
  1. PXD037659
     1. Label: PRIDE project
     2. Name: Effect of knockdEffect of knockdown of mitochondrial atp1 mRNA by a custom-designed PPR protein on protein expression in Arabidopsis thaliana own of mitochondrial atp1 mRNA by a custom-designed PPR protein on protein expression in Arabidopsis thaliana