PXD037649

PXD037649 is an original dataset announced via ProteomeXchange.

Title	Proteome comparison of a new human cholangiocarcinoma 3D co-culture tumor model to 3D single cultures.
Description	Cholangiocarcinomas (CCA) are characterized by their desmoplastic and hypervascularized tumor microenvironment (TME) which is mainly composed of tumor cells and cancer-associated fibroblasts (CAFs). CAFs play a pivotal role in tumor progression, angiogenesis, metastasis and the development of therapy resistance. To our knowledge, no continuous human in vivo-like co-culture model is available for research. Therefore, we aimed to establish a new model that mimics the desmoplastic environment typically seen in CCA. Proteomic data comparing the new CCA tumor cell line with our co-culture tumor model (CCTM) indicated a higher correlation of the CCTM with physiological CCA characteristics according to literature. A pro-angiogenic TME that is typically observed in CCA could also be simulated in the CCTM group. Further analysis of secreted proteins revealed CAFs to be the main source for these angiogenic factors. Our CCTM represents a new, reproducible and easy-to-handle 3D CCA model for preclinical studies focusing on CCA-stromal crosstalk, tumor angiogenesis and invasion, the immunosuppressive microenvironment and the involvement of CAFs in the way that drug resistance develops.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:50.025.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Liesinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-24 00:55:23	ID requested
1	2023-05-10 15:33:24	announced
⏵ 2	2023-11-14 08:47:56	announced	2023-11-14: Updated project metadata.

Schrom S, Kleinegger F, Anders I, Hebesberger T, Karner C, Liesinger L, Birner-Gruenberger R, Renner W, Pichler M, Grillari R, Aigelsreiter A, Rinner B, MUG CCArly: A Novel Autologous 3D Cholangiocarcinoma Model Presents an Increased Angiogenic Potential. Cancers (Basel), 15(6):(2023) [pubmed]

submitter keyword: Human, DIA, cholangiocarcinoma, 3D cell culture, LC-MS/MS

Ruth Birner-Gruenberger
contact affiliation	Faculty of Technical Chemistry, Institute of Chemical Technologies and Analytics, Technische Universität Wien (TU Wien), 1060 Vienna, Austria; Diagnostic and Research Institute of Pathology, Medical University of Graz, 8036 Graz, Austria; BiotechMed-Graz, 8010 Graz, Austria;
contact email	ruth.birner-gruenberger@tuwien.ac.at
lab head
Laura Liesinger
contact affiliation	Faculty of Technical Chemistry, Institute of Chemical Technologies and Analytics, Technische Universität Wien (TU Wien), 1060 Vienna, Austria;
contact email	laura.liesinger@tuwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037649

PRIDE project URI

• PRIDE
  1. PXD037649
     1. Label: PRIDE project
     2. Name: Proteome comparison of a new human cholangiocarcinoma 3D co-culture tumor model to 3D single cultures.