PXD037644
PXD037644 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Motif-targeting phosphoproteome analysis of cancer cells for profiling kinase inhibitors. |
Description | We developed a motif-enriched phosphoproteome analysis workflow utilizing an in vitro kinase reaction to enrich a set of peptides with specific primary sequence motifs. The kinase reaction attached the phosphate tag to the peptides derived from phosphopeptide enrichment and subsequent dephosphorylation, facilitates the enrichment of the kinase substrates from phosphoproteomic samples. We applied this method to phosphoproteomic profiling of inhibitor treated cancer cells, and successfully profiled kinase inhibitors with different inhibitory spectra. We anticipate this workflow will be useful to target a specific set of the phosphoproteome with the wide variety of available recombinant protein kinases. |
HostingRepository | jPOST |
AnnounceDate | 2022-12-23 |
AnnouncementXML | Submission_2022-12-22_16:32:04.727.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kosuke Ogata |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; alpha-amino acetylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
Instrument | Q Exactive; TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-10-22 09:04:50 | ID requested | |
⏵ 1 | 2022-12-22 16:32:05 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Phosphoproteomics, kinase inhibitor, in vitro kinase reaction |
Contact List
Yasushi Ishihama | |
---|---|
lab head | |
Kosuke Ogata | |
contact affiliation | Kyoto university |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001896/ |