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PXD037644

PXD037644 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMotif-targeting phosphoproteome analysis of cancer cells for profiling kinase inhibitors.
DescriptionWe developed a motif-enriched phosphoproteome analysis workflow utilizing an in vitro kinase reaction to enrich a set of peptides with specific primary sequence motifs. The kinase reaction attached the phosphate tag to the peptides derived from phosphopeptide enrichment and subsequent dephosphorylation, facilitates the enrichment of the kinase substrates from phosphoproteomic samples. We applied this method to phosphoproteomic profiling of inhibitor treated cancer cells, and successfully profiled kinase inhibitors with different inhibitory spectra. We anticipate this workflow will be useful to target a specific set of the phosphoproteome with the wide variety of available recombinant protein kinases.
HostingRepositoryjPOST
AnnounceDate2022-12-23
AnnouncementXMLSubmission_2022-12-22_16:32:04.727.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKosuke Ogata
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; alpha-amino acetylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
InstrumentQ Exactive; TripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-22 09:04:50ID requested
12022-12-22 16:32:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Phosphoproteomics, kinase inhibitor, in vitro kinase reaction
Contact List
Yasushi Ishihama
lab head
Kosuke Ogata
contact affiliationKyoto university
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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