PXD037639

PXD037639 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of serial-cultured human iPSC-derived MSC with lamin A/C mutation
Description	Pathogenic mutations in lamin A/C (LMNA) lead to nuclear structural abnormalities, mesenchymal tissue damage, and laminopathies, which have numerous tissue-specific and progeria phenotypes. However, how LMNA mutations lead to accelerated mesenchymal-derived cell senescence remains unclear. Here, we established a replicative senescence model in vitro using induced pluripotent stem cell-derived mesenchymal stem cells (iMSCs) from patients with homozygous LMNA p.R527C mutation (LMNA R527C iMSCs). R527C iMSCs exhibited marked cell senescence and stemness potential attenuation, accompanied by immunophenotypic changes when expanded to passage 13 in vitro. Proteome analysis revealed that DNA replication, nuclear structure, and chromatin-related gene sets were the most significant changes in R527C iMSCs during replicative senescence, and pathways such as cell cycle, DNA replication, cell adhesion, and inflammation might play important roles in senescence.
HostingRepository	iProX
AnnounceDate	2022-10-21
AnnouncementXML	Submission_2022-10-21_01:59:15.422.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liangge He
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	methylthiolated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-21 01:58:58	ID requested
⏵ 1	2022-10-21 01:59:15	announced

Dataset with its publication pending

submitter keyword: Human, MSC, LMNA

Liangge He
contact affiliation	Shenzhen University
contact email	liangge_he@163.com
lab head
Liangge He
contact affiliation	Shenzhen University
contact email	liangge_he@163.com
dataset submitter

iProX dataset URI