PXD037632

PXD037632 is an original dataset announced via ProteomeXchange.

Title	Comparative single-cell transcriptional and proteomic atlas of clinical-grade injectable mesenchymal source tissues
Description	Bone marrow aspirate concentrate (BMAC) and adipose-derived stromal vascular fraction (ADSVF) are the most marketed stem cell therapies to treat a variety of conditions in the general population and elite athletes. Both tissues have been used interchangeably clinically even though their detailed composition, heterogeneity, and mechanisms of action have neither been rigorously inventoried nor compared. This lack of information has prevented investigations into ideal dosages and has facilitated anecdata and misinformation. Here, we analyzed single-cell transcriptomes, proteomes, and flow cytometry profiles from paired clinical-grade BMAC and ADSVF. This comparative transcriptional atlas challenges the prevalent notion that there is one therapeutic cell type present in both tissues. We also provide data of surface markers that may enable isolation and investigation of cell (sub)populations. Furthermore, the proteome atlas highlights intertissue and interpatient heterogeneity of injected proteins with potentially regenerative or immunomodulatory capacities.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:51:26.748.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samuel Ward
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-20 13:52:00	ID requested
1	2024-07-25 10:11:10	announced
⏵ 2	2024-10-22 06:51:27	announced	2024-10-22: Updated project metadata.

10.1126/SCIADV.ADN2831;

submitter keyword: Human, LC-MSMS, bone marrow, adipose, BMAC, ADSVF

Samuel Richard
contact affiliation	Professor, Departments of Orthopaedic Surgery, Radiology, and Bioengineering Vice Chair of Research and Innovation (Orthopaedic Surgery) UC San Diego 9500 Gilman Drive (0863) La Jolla, CA 92093-0863 USA
contact email	s1ward@health.ucsd.edu
lab head
Samuel Ward
contact affiliation	UC San Diego
contact email	s1ward@health.ucsd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD037632

PRIDE project URI

• PRIDE
  1. PXD037632
     1. Label: PRIDE project
     2. Name: Comparative single-cell transcriptional and proteomic atlas of clinical-grade injectable mesenchymal source tissues