PXD037620

PXD037620 is an original dataset announced via ProteomeXchange.

Title	RBPMS mediates smooth muscle specific alternative splicing via oligomerization and modulation of trans splicing factor recruitment
Description	RBPMS regulates alternative splicing decision via recruiting other trans factors. This experiment aims to investigate the interactome of Strep-II tagged recombinant RBPMS in Hela nuclear extract. The interactome of the full length protein is compared to the construct lacking the far C-terminal 20 amino acids. The “R” series indicates full-length RBPMS pull-down. The “P” series indicates truncated RBPMS pull-down. The “BL” series indicates full length RBPMS pull-down in the absence of nuclear extract input but with bovine serum albumin beads blocking reagent. The “N” series indicates non-baited negative control pull-down.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:22.954.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-20 13:29:20	ID requested
1	2023-10-24 11:19:25	announced
⏵ 2	2023-11-14 09:07:30	announced	2023-11-14: Updated project metadata.

Yang Y, Lee GC, Nakagaki-Silva E, Huang Y, Peacey M, Partridge R, Gooding C, Smith CWJ, Cell-type specific regulator RBPMS switches alternative splicing via higher-order oligomerization and heterotypic interactions with other splicing regulators. Nucleic Acids Res, 51(18):9961-9982(2023) [pubmed]

submitter keyword: Human

LC MSMS

RBPMS

AP-MS: splicing factor

RBP

Chris WJ Smith
contact affiliation	Department of Biochemistry, University of Cambridge
contact email	cwjs1@cam.ac.uk
lab head
Yi Yang
contact affiliation	Department of Biochemistry, University of Cambridge
contact email	yy462@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD037620

PRIDE project URI

• PRIDE
  1. PXD037620
     1. Label: PRIDE project
     2. Name: RBPMS mediates smooth muscle specific alternative splicing via oligomerization and modulation of trans splicing factor recruitment