PXD037612

PXD037612 is an original dataset announced via ProteomeXchange.

Title	Argiope (Orb weaver) aggregate glue protein composition
Description	Using proteomics, we documented the glue composition in two congeners that live in different environments, Argiope argentata (dry southwest US) and A. trifasciata (humid southeast US). The viscoelastic protein cores of A. argentata droplets comprised a smaller portion of droplet volume than did those of A. trifasciata and, as humidity increased, incorporated a smaller percentage of absorbed water. Argiope argentata core protein was many times stiffer and tougher than A. trifasciata protein. Each species’ glue included ~30 aggregate-expressed proteins, most of which (24 and 23, respectively) were homologous between the two species. However, the relative contribution and number of gene family members of each homologous group differed. For instance, the aggregate spidroins (AgSp1 and AgSp2) accounted for nearly half of the detected glue composition in A. argentata, but only 38% in A. trifasciata. Additionally, AgSp1, which has highly negatively charged regions, was ~2X as abundant as the positively charged AgSp2 in A. argentata but ~3X as abundant in A. trifasciata. As another example, A. argentata glue included 11 members of a newly discovered cysteine-rich gene family, versus 5 in A. trifasciata. The ability to selectively express different glue protein genes and/or to extrude their products at different rates provides a faster mechanism to evolve material properties than sequence evolution alone.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:53:53.447.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Clarke
SpeciesList	scientific name: Argiope argentata; NCBI TaxID: 233271; scientific name: Argiope trifasciata; NCBI TaxID: 156845;
ModificationList	phosphorylated residue; monohydroxylated residue; Hex-HexNAc
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-20 13:26:06	ID requested
1	2023-07-13 06:17:20	announced
2	2023-11-14 07:11:27	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:53:53	announced	2024-10-22: Updated project metadata.

10.3389/FEVO.2023.1099481;

submitter keyword: Spider, glue, Argiope

Nadia Ayoub
contact affiliation	Department of Biology, Washington and Lee University
contact email	nayoub@wlu.edu
lab head
Thomas Clarke
contact affiliation	Washington and Lee
contact email	tclarke@wlu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037612
     1. Label: PRIDE project
     2. Name: Argiope (Orb weaver) aggregate glue protein composition