PXD037612 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Argiope (Orb weaver) aggregate glue protein composition |
Description | Using proteomics, we documented the glue composition in two congeners that live in different environments, Argiope argentata (dry southwest US) and A. trifasciata (humid southeast US). The viscoelastic protein cores of A. argentata droplets comprised a smaller portion of droplet volume than did those of A. trifasciata and, as humidity increased, incorporated a smaller percentage of absorbed water. Argiope argentata core protein was many times stiffer and tougher than A. trifasciata protein. Each species’ glue included ~30 aggregate-expressed proteins, most of which (24 and 23, respectively) were homologous between the two species. However, the relative contribution and number of gene family members of each homologous group differed. For instance, the aggregate spidroins (AgSp1 and AgSp2) accounted for nearly half of the detected glue composition in A. argentata, but only 38% in A. trifasciata. Additionally, AgSp1, which has highly negatively charged regions, was ~2X as abundant as the positively charged AgSp2 in A. argentata but ~3X as abundant in A. trifasciata. As another example, A. argentata glue included 11 members of a newly discovered cysteine-rich gene family, versus 5 in A. trifasciata. The ability to selectively express different glue protein genes and/or to extrude their products at different rates provides a faster mechanism to evolve material properties than sequence evolution alone. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:53:53.447.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Clarke |
SpeciesList | scientific name: Argiope argentata; NCBI TaxID: 233271; scientific name: Argiope trifasciata; NCBI TaxID: 156845; |
ModificationList | phosphorylated residue; monohydroxylated residue; Hex-HexNAc |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-20 13:26:06 | ID requested | |
1 | 2023-07-13 06:17:20 | announced | |
2 | 2023-11-14 07:11:27 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 05:53:53 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Spider, glue, Argiope |
Contact List
Nadia Ayoub |
contact affiliation | Department of Biology, Washington and Lee University |
contact email | nayoub@wlu.edu |
lab head | |
Thomas Clarke |
contact affiliation | Washington and Lee |
contact email | tclarke@wlu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037612
- Label: PRIDE project
- Name: Argiope (Orb weaver) aggregate glue protein composition