<<< Full experiment listing

PXD037611

PXD037611 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAmmonium Sulfate-based Prefractionation Improved Carbonylated Proteome Coverage in Arabidopsis thaliana Leaf Extract
DescriptionThe hypothesis that ammonium sulfate-based protein precipitation can help prefractionate the total protein extract into fractions of reduced complexity and improve the detection of low-abundance proteins was investigated. This dataset resulted from the experiments that we performed to improve the detection of the carbonylated proteins in leaf extract. A total of 122 carbonylated proteins were identified, of which 77 were found in the ammonium sulfate fractions only. This prefractionation method allowed to identify 63% more carbonylated proteins compared to the number of carbonylated proteins identified from the total crude extract without prefractionation. Our results thus showed that a simple offline prefractionation of the proteome by ammonium sulfate prior to liquid chromatography-tandem mass spectrometry significantly improved the detection of carbonylated proteins in Arabidopsis leaf extract.
HostingRepositoryMassIVE
AnnounceDate2023-02-19
AnnouncementXMLSubmission_2023-02-19_10:44:13.059.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTagnon Missihoun
SpeciesList scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-20 11:25:25ID requested
12023-02-19 10:44:13announced
Publication List
no publication
Keyword List
submitter keyword: Aldehyde reactive probe, peptide level enrichment, protein carbonylation, proteome offline prefractionation
Contact List
Tagnon Missihoun
contact affiliationUniversity of Quebec Trois-Rivieres
contact emailTagnon.Missihoun@uqtr.ca
lab head
Tagnon Missihoun
contact affiliationUniversity of Quebec Trois-Rivieres
contact emailTagnon.Missihoun@uqtr.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090559/