PXD037597

PXD037597 is an original dataset announced via ProteomeXchange.

Title	OsRALF17/19 activates ROS signaling through OsMTD2 to modulate pollen tube growth in Oryza sativa
Description	- Pollen tube growth is important process for successful double fertilization, which is critical for grain yield in crop plants. Despite much progress in identification of rapid alkalization factors (RALFs) which serve as ligand for signaling transduction during fertilization in Arabidopsis, there is no functional study of RALF in mono-cotyledon plant. - We functionally characterized two pollen specific RALF in rice (Oryza sativa) using multiple CRISPR/Cas9 induced loss-of-function mutants, peptide treatment, expression analyses, tag reporter lines. - OsRALF17 is specifically expressed in pollen and pollen tube as the strongest level among 41 RALF members in rice. Exogenously applied OsRALF17 inhibits pollen tube germination and elongation at high concentration, but enhances tube elongation at low concentration, indicating the regulation of growth balance. Double mutant of OsRALF17 with OsRALF19 exhibit almost male sterile, with defect on pollen germination and tube elongation. - Our study revealed that functionally-redundant OsRALF17 and 19 peptides binds to the OsMTD2, CrRLK1L family member, and transmits ROS signal for pollen tube germination and integrity maintenance in rice. We provide new insights into the role of RALF and expanding our understanding of the biological role of RALF in regulating rice fertilization.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:03:36.442.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Tae Kim
SpeciesList	scientific name: Oryza sativa subsp. japonica (Rice); NCBI TaxID: 39947;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-20 02:22:28	ID requested
1	2023-09-20 22:21:14	announced
2	2023-11-14 09:05:21	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:03:37	announced	2024-10-22: Updated project metadata.

Kim EJ, Kim JH, Hong WJ, Kim EY, Kim MH, Lee SK, Min CW, Kim ST, Park SK, Jung KH, Kim YJ, Rice pollen-specific OsRALF17 and OsRALF19 are essential for pollen tube growth. J Integr Plant Biol, 65(9):2218-2236(2023) [pubmed]

10.1111/jipb.13508;

submitter keyword: Oryza sativa, Monocot, rapid alkalization factor (RALF),CRISPR-Cas9, pollen tube growth, CrRLK1L

Yu-Jin Kim
contact affiliation	Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea
contact email	yjkim2020@pusan.ac.kr
lab head
Sun Tae Kim
contact affiliation	Pusan National University
contact email	stkim5505@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037597
     1. Label: PRIDE project
     2. Name: OsRALF17/19 activates ROS signaling through OsMTD2 to modulate pollen tube growth in Oryza sativa