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PXD037597

PXD037597 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOsRALF17/19 activates ROS signaling through OsMTD2 to modulate pollen tube growth in Oryza sativa
Description- Pollen tube growth is important process for successful double fertilization, which is critical for grain yield in crop plants. Despite much progress in identification of rapid alkalization factors (RALFs) which serve as ligand for signaling transduction during fertilization in Arabidopsis, there is no functional study of RALF in mono-cotyledon plant. - We functionally characterized two pollen specific RALF in rice (Oryza sativa) using multiple CRISPR/Cas9 induced loss-of-function mutants, peptide treatment, expression analyses, tag reporter lines. - OsRALF17 is specifically expressed in pollen and pollen tube as the strongest level among 41 RALF members in rice. Exogenously applied OsRALF17 inhibits pollen tube germination and elongation at high concentration, but enhances tube elongation at low concentration, indicating the regulation of growth balance. Double mutant of OsRALF17 with OsRALF19 exhibit almost male sterile, with defect on pollen germination and tube elongation. - Our study revealed that functionally-redundant OsRALF17 and 19 peptides binds to the OsMTD2, CrRLK1L family member, and transmits ROS signal for pollen tube germination and integrity maintenance in rice. We provide new insights into the role of RALF and expanding our understanding of the biological role of RALF in regulating rice fertilization.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:03:36.442.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSun Tae Kim
SpeciesList scientific name: Oryza sativa subsp. japonica (Rice); NCBI TaxID: 39947;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-20 02:22:28ID requested
12023-09-20 22:21:14announced
22023-11-14 09:05:21announced2023-11-14: Updated project metadata.
32024-10-22 06:03:37announced2024-10-22: Updated project metadata.
Publication List
Kim EJ, Kim JH, Hong WJ, Kim EY, Kim MH, Lee SK, Min CW, Kim ST, Park SK, Jung KH, Kim YJ, Rice pollen-specific OsRALF17 and OsRALF19 are essential for pollen tube growth. J Integr Plant Biol, 65(9):2218-2236(2023) [pubmed]
10.1111/jipb.13508;
Keyword List
submitter keyword: Oryza sativa, Monocot, rapid alkalization factor (RALF),CRISPR-Cas9, pollen tube growth, CrRLK1L
Contact List
Yu-Jin Kim
contact affiliationDepartment of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea
contact emailyjkim2020@pusan.ac.kr
lab head
Sun Tae Kim
contact affiliationPusan National University
contact emailstkim5505@gmail.com
dataset submitter
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Dataset FTP location
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