PXD037594

PXD037594 is an original dataset announced via ProteomeXchange.

Title	Inhibitors molecules isolated from midgut and midgut diverticula of the spider Nephilingis cruentata
Description	Spiders are insect predators and their venoms are important to prey capture. Spider venomshave several potential applications as pharmaceutical compounds and insecticides. However,transcriptomic and proteomic analyses of the digestive system (DS) of spiders show that DS is also a rich source of new inhibitor molecules. Biochemical, transcriptomic and proteomic data of crude DS extracts show the presence of molecules with inhibitor potential in the spider Nephilingis cruentata. Therefore, the aims of this work were to isolate and characterize molecules with serine peptidase inhibitory activity. The DS of fasting adult females was homogenized under acidic conditions and subjected to heat treatment. After that, samples were submitted to ion exchange batch and high-performance reverse-phase chromatography. The fractions with inhibitory activity were confirmed by mass spectrometry, identifying 6 molecules with inhibitor potential. The inhibitor Nc6834 was kinetically characterized, showing a KD value of 30.25 nM ± 8.13. Analysis of the tertiary structure by molecular modeling using Alpha-Fold2 indicates that the inhibitor Nc6834 structurally belongs to theMIT1-like atracotoxin family. This is the first time that a serine peptidase inhibitory function is attributed to this structural family and the reactive site inhibitor residue is identified. Sequence analysis indicates that these molecules may be present in the DS of other spiders.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:23:57.027.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037594
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Oscar Bento
SpeciesList	scientific name: Nephilengys cruentata; NCBI TaxID: 450950;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Dionex instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-20 02:17:49	ID requested
1	2023-03-10 16:59:16	announced
⏵ 2	2023-11-14 08:23:57	announced	2023-11-14: Updated project metadata.

Neto OBS, Vallad, ã, o R, Coelho GR, Dias R, Pimenta DC, Lopes AR, Spiders' digestive system as a source of trypsin inhibitors: functional activity of a member of atracotoxin structural family. Sci Rep, 13(1):2389(2023) [pubmed]

10.6019/PXD037594;

submitter keyword: Inhibitors. Spider. Midgut. Midgut diverticula

Adriana Rios Lopes
contact affiliation	Instituto Butantan, Laboratory of Biochemistry, São Paulo,05503900, Brazil
contact email	adriana.lopes@butantan.gov.br
lab head
Oscar Bento
contact affiliation	Universidade de São Paulo, ICB.
contact email	oscar.bentoneto@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037594

PRIDE project URI

• PRIDE
  1. PXD037594
     1. Label: PRIDE project
     2. Name: Inhibitors molecules isolated from midgut and midgut diverticula of the spider Nephilingis cruentata