PXD037586

PXD037586 is an original dataset announced via ProteomeXchange.

Title	nitric oxide-reducing microbial community metaproteomics
Description	Here, we successfully used NO as the direct electron acceptor for the enrichment of a microbial community in a continuous bioreactor. The enrichment culture, mainly comprised of two new organisms from the Sterolibacteriaceae family, grew on NO reduction to N2 and formate oxidation, with virtually no accumulation of N2O. The microbial growth kinetics of the enrichment culture as well as its affinity for different N-oxides were determined. In parallel, using metagenomics, metatranscriptomics, and metaproteomics, the biochemical reactions underlying the growth of these microorganisms on NO were investigated. This study demonstrates that microorganisms thrive and can be enriched on NO, and presents new opportunities to study microbial growth on this highly energetic and climate-active molecule that may have been pivotal in the evolution of aerobic respiration.
HostingRepository	PRIDE
AnnounceDate	2023-06-09
AnnouncementXML	Submission_2023-06-09_02:59:57.794.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HansWessels
SpeciesList	scientific name: Sterolibacteriaceae; NCBI TaxID: 2008793;
ModificationList	monohydroxylated residue; formylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-20 02:03:43	ID requested
⏵ 1	2023-06-09 02:59:58	announced

Dataset with its publication pending

submitter keyword: metaproteomics

HansWessels
contact affiliation	Translational Metabolic Laboratory, Radboudumc, Nijmegen, The Netherlands
contact email	hans.wessels@radboudumc.nl
lab head
HansWessels
contact affiliation	Radboudumc
contact email	hans.wessels@radboudumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD037586

PRIDE project URI

• PRIDE
  1. PXD037586
     1. Label: PRIDE project
     2. Name: nitric oxide-reducing microbial community metaproteomics