PXD037583

PXD037583 is an original dataset announced via ProteomeXchange.

Title	Peroxynitrite-mediated post-translational modification of human glutamine synthetase
Description	The aim of the project was to understand the mechanisms that underlie the oxidative inactivation of human glutamine synthetase (HsGS). For that purpose, we expressed and purified recombinant HsGS, exposed it in phosphate buffer to peroxynitrite (ONOO-) and assessed the functional and structural consequences. To characterize the oxidative post-translational modifications induced by ONOO-, several approaches were carried out (e.g. SDS-PAGE, western-blot, amino acid quantification by LC, etc). The most detailed characterization was performed through LC-MS/MS analysis after trypsin digestion of the ONOO--treated protein, which allowed us to identify the amino acid residues that are modified and to estimate the extent of such modifications.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:48.845.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolás Campolo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	3'-nitro-L-tyrosine; (18)O label at both C-terminal oxygens; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-20 02:02:28	ID requested
1	2023-05-10 13:37:42	announced
⏵ 2	2023-11-14 08:46:49	announced	2023-11-14: Updated project metadata.

Campolo N, Mastrogiovanni M, Mariotti M, Issoglio FM, Estrin D, H, ä, gglund P, Grune T, Davies MJ, Bartesaghi S, Radi R, Multiple oxidative post-translational modifications of human glutamine synthetase mediate peroxynitrite-dependent enzyme inactivation and aggregation. J Biol Chem, 299(3):102941(2023) [pubmed]

submitter keyword: Peroxynitrite, glutamine synthetase, oxidative stress, protein oxidation, nitrotyrosine

Rafael Radi
contact affiliation	Departamento de Bioquímica y Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
contact email	rradi@fmed.edu.uy
lab head
Nicolás Campolo
contact affiliation	Departamento de Bioquímica & Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina, Universidad de la República, Uruguay
contact email	ncampolo@fmed.edu.uy
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037583

PRIDE project URI

• PRIDE
  1. PXD037583
     1. Label: PRIDE project
     2. Name: Peroxynitrite-mediated post-translational modification of human glutamine synthetase