PXD037582

PXD037582 is an original dataset announced via ProteomeXchange.

Title	Uncovering structural themes across cilia microtubule inner proteins with implications for human cilia function
Description	Centrosomes and cilia are microtubule-based superstructures vital for cell division, signaling, and motility. The once thought hollow lumen of their microtubule core structures was recently found to hold a rich meshwork of microtubule inner proteins (MIPs). To address outstanding questions on how distinct MIPs evolved to recognize microtubule inner surfaces, we applied computational sequence analyses, structure predictions, and experimental validation to uncover evolutionarily conserved microtubule- and MIP-binding domains named NWE, PYG, SNYG, ELLEn, and GFG-repeat by their signature motifs. These novel domains intermix with MT-binding DM10-modules and Mn-repeats in 24 Chlamydomonas and 33 human proteins. The domains specialties provided keys to identify elusive cross-species homologs, 11 hitherto unknown human MIP candidates, and functional properties for five protein subfamilies, including the microtubule seam-binding NWE and ELLEn families. Our work demonstrates that MIPs co-evolved with centrosomes and cilia, defines structural innovations that underpin centriole and axoneme assembly, and explain causes of ciliopathies.
HostingRepository	PRIDE
AnnounceDate	2024-03-01
AnnouncementXML	Submission_2024-03-01_00:16:37.491.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037582
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jens Andersen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-20 02:02:04	ID requested
⏵ 1	2024-03-01 00:16:37	announced

10.6019/PXD037582;

submitter keyword: cilia, centrosomes, proteomics, sequence alignment, microtubules,Microtubule inner proteins, Alphafold2

Jens A. Andersen
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
contact email	jens.andersen@bmb.sdu.dk
lab head
Jens Andersen
contact affiliation	University of Southern Denmark, Department og Biochemistry and Molecular Biology
contact email	jens.andersen@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD037582

PRIDE project URI

• PRIDE
  1. PXD037582
     1. Label: PRIDE project
     2. Name: Uncovering structural themes across cilia microtubule inner proteins with implications for human cilia function