PXD037562

PXD037562 is an original dataset announced via ProteomeXchange.

Title	Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release - table s1 corrected
Description	Ribosome synthesis begins in the nucleolus with 90S pre-ribosome construction, but little is known about how the many different snoRNAs that modify the pre-rRNA are timely guided to their target sites. Here, we report a role for Cms1 in such a process. Initially, we discover CMS1 as null suppressor of a nop14 mutant impaired in Rrp12–Enp1 factor recruitment to the 90S. Further investigations detect Cms1 at the 18S rRNA 3'-major domain of an early 90S that carries H/ACA snR83, known to guide pseudouridylation at two target sites within the same subdomain. Cms1 co-precipitates with many 90S factors, but Rrp12-Enp1 encircling the 3'-major domain in the mature 90S is decreased. We suggest that Cms1 associates with the 3’-major domain during early 90S biogenesis to restrict premature Rrp12–Enp1 binding, but allows snR83 to timely perform its modification role before the next 90S assembly steps coupled with Cms1 release take place.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:40:12.284.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037562
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Benjamin Lau
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-19 03:05:12	ID requested
1	2023-03-11 01:58:49	announced
⏵ 2	2023-11-14 08:40:13	announced	2023-11-14: Updated project metadata.

Lau B, Beine-Golovchuk O, Kornprobst M, Cheng J, Kressler D, J, á, dy B, Kiss T, Beckmann R, Hurt E, Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release. Cell Rep, 41(8):111684(2022) [pubmed]

10.6019/PXD037562;

submitter keyword: Ribosome biogenesis, 90S pre-ribosome, snoRNA, Cms1

Prof Dr Ed Hurt
contact affiliation	Biochemistry Center Heidelberg University of Heidelberg Germany
contact email	ed.hurt@bzh.uni-heidelberg.de
lab head
Benjamin Lau
contact affiliation	Heidelberg University
contact email	benjamin.lau@bzh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037562

PRIDE project URI

• PRIDE
  1. PXD037562
     1. Label: PRIDE project
     2. Name: Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release - table s1 corrected