PXD037561

PXD037561 is an original dataset announced via ProteomeXchange.

Title	The claudin-like apicomplexan microneme protein is essential for Plasmodium berghei sporozoite infectivity in the mosquito and mammalian hosts
Description	Here we characterize the function of a microneme protein expressed both in merozoites and sporozoites, the claudin-like protein CLAMP. We used a conditional genome editing strategy in a rodent malaria model to generate CLAMP-deficient sporozoites. In the absence of CLAMP, sporozoites failed to invade mosquito salivary glands and mammalian hepatocytes, and showed defects in gliding motility and microneme secretion. Our data establish that CLAMP plays an essential role across Plasmodium invasive stages, and might represent a potential target for transmission-blocking antimalarial strategies.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:36.064.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soumia HAMADA
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-19 02:58:59	ID requested
1	2023-05-10 13:21:21	announced
⏵ 2	2023-11-14 08:46:44	announced	2023-11-14: Updated project metadata.

Loubens M, Marinach C, Paquereau CE, Hamada S, Hoareau-Coudert B, Akbar D, Franetich JF, Silvie O, The claudin-like apicomplexan microneme protein is required for gliding motility and infectivity of Plasmodium sporozoites. PLoS Pathog, 19(3):e1011261(2023) [pubmed]

submitter keyword: Plasmodium

malaria

sporozoites

conditional mutagenesis

CLAMP

Solenne Chardonnet
contact affiliation	Sorbonne Université, INSERM, UMS PASS, Plateforme Post-génomique de la Pitié Salpêtrière (P3S), 75013, Paris, France
contact email	solenne.chardonnet@sorbonne-universite.fr
lab head
Soumia HAMADA
contact affiliation	Plateforme P3S UMS-37 PASS – Sorbonne Universite
contact email	soumia.hamada@sorbonne-universite.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037561

PRIDE project URI

• PRIDE
  1. PXD037561
     1. Label: PRIDE project
     2. Name: The claudin-like apicomplexan microneme protein is essential for Plasmodium berghei sporozoite infectivity in the mosquito and mammalian hosts