PXD037531

PXD037531 is an original dataset announced via ProteomeXchange.

Title	High resolution human sperm proteome LC-MSMS
Description	The aim of this study was to achieve a higher resolution of the human sperm proteome for better understanding of the molecular causes of male infertility. For this purpose, we examined the proteomic profiles of sperm from 76 men for proteins with deregulated abundances. Infertile men had abnormal semen parameters and were involuntarily childless. Men in the cohort with normal fertility had normozoospermia and had fathered children without medical assistance. Mass spectrometry analysis led to an in-depth reconstruction of the human sperm proteome. The corresponding genes were mainly involved in cellular motility, response to stimuli, adhesion, and reproduction. We also observed increasing numbers of at least three-fold differentially abundant sperm proteins from oligozoospermia and oligoasthenozoospermia to oligoasthenoteratozoospermia, compared with normozoospermia. Primarily, flagellar assembly and sperm motility, sperm-egg interaction, and male gametogenesis were affected. This suggests that sperm of infertile men carry signatures of impaired sperm production and are functionally impaired. Not least, we present new male infertility markers in addition to established ones.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:45:27.625.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Dejung
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-18 08:11:54	ID requested
1	2023-04-11 08:30:53	announced
⏵ 2	2023-11-14 06:45:28	announced	2023-11-14: Updated project metadata.

Greither T, Dejung M, Behre HM, Butter F, Herlyn H, The human sperm proteome-Toward a panel for male fertility testing. Andrology, 11(7):1418-1436(2023) [pubmed]

submitter keyword: Human, LC-MSMS, sperm

Holger Herlyn
contact affiliation	Institute of Organismic and Molecular Evolution (iomE), Anthropology Johannes Gutenberg-Universität Mainz | JGU Germany
contact email	hherlyn@uni-mainz.de
lab head
Mario Dejung
contact affiliation	Institut of Molecular Biology
contact email	m.dejung@imb.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD037531

PRIDE project URI

• PRIDE
  1. PXD037531
     1. Label: PRIDE project
     2. Name: High resolution human sperm proteome LC-MSMS